Song Yang, Zhao Shupeng, Qi Fengjie, Zheng Huachuan
Department of Pathology, First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121001, China.
Department of Thyroid Surgery, First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121001, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Jan;34(1):53-58.
Objective To investigate the nucleocytoplasmic translocation of inhibitor of growth family member 5 (ING5) gene in human breast cancer and the negative correlation between the nuclear high expression of ING5 protein and the clinicopathological characteristics of breast cancer. Methods We collected 260 cases of clinical primary breast cancer, 55 cases of metastatic cancer in lymph node, 61 cases of breast fibroadenoma, 110 cases of adenomatosis, and 91 cases of para-cancerous tissues. With the tissue microarrays, we detected ING5 expression in the nucleus and cytoplasm through immunohistochemistry, and statistically analyzed the correlations of nuclear and cytoplasmic ING5 expression with the clinicopathological characteristics of breast cancer. Results The expression of nuclear ING5 in the para-cancerous tissues was obviously higher than that in the fibroadenoma, adenomatosis and primary breast cancer; it was higher in the adenomatosis than in the primary breast cancer, and higher in the primary breast cancer than in the metastatic cancer in lymph node. The cytoplasmic ING5 expression in the para-carcinoma tissue was higher than that in the fibroadenoma, adenomatosis and primary breast cancer. These data suggested the nucleocytoplasmic translocation of ING5 protein in breast cancer. Moreover, nuclear ING5 high expression was negatively correlated with distant metastasis and positively with P53 expression, while cytoplasmic ING5 high expression was positively correlated with tumor size and estrogen receptor expression. The cytoplasm ING5 expression of triple-negative breast cancer was lower than that in the non-triple-negative breast cancer. Conclusion Nucleocytoplasmic translocation of ING5 protein occurs in breast cancer, and the high expression of nuclear ING5 is inversely related to some poor clinicopathological behaviors of breast cancer.
目的 探讨生长抑制因子家族成员5（ING5）基因在人乳腺癌中的核质转运情况以及ING5蛋白核高表达与乳腺癌临床病理特征的负相关关系。方法 收集260例临床原发性乳腺癌、55例淋巴结转移癌、61例乳腺纤维瘤、110例腺病及91例癌旁组织。采用组织芯片，通过免疫组化检测ING在细胞核和细胞质中的表达，并对细胞核和细胞质中ING5表达与乳腺癌临床病理特征的相关性进行统计学分析。结果 癌旁组织中ING5的核表达明显高于纤维瘤、腺病和原发性乳腺癌；腺病中的核表达高于原发性乳腺癌，原发性乳腺癌中的核表达高于淋巴结转移癌。癌旁组织中ING5的细胞质表达高于纤维瘤、腺病和原发性乳腺癌。这些数据提示乳腺癌中ING5蛋白存在核质转运。此外，ING5核高表达与远处转移呈负相关，与P53表达呈正相关，而ING5细胞质高表达与肿瘤大小和雌激素受体表达呈正相关。三阴性乳腺癌的细胞质ING5表达低于非三阴性乳腺癌。结论 乳腺癌中存在ING5蛋白的核质转运，ING5核高表达与乳腺癌某些不良临床病理行为呈负相关。
