Maclellan Reid A, Konczyk Dennis J, Goss Jeremy A, Greene Arin K
From the Department of Plastic and Oral Surgery, Vascular Anomalies Center, Boston Children's Hospital, Harvard Medical School, Boston, MA.
Ann Plast Surg. 2018 Apr;80(4 Suppl 4):S211-S213. doi: 10.1097/SAP.0000000000001438.
The life cycle of infantile hemangioma (IH) and secretion of follicle-stimulating hormone (FSH) are identical. We previously have shown that IH contains the FSH receptor (FSHR). The purpose of this study was to identify which cell type(s) in IH expresses FSHR.
Human proliferating IH tissues obtained during a clinically indicated surgical procedure were used. Paraffin sections and isolated cell populations (endothelial, pericyte, stem cell) were subjected to immunofluorescence for FSHR. Tissues were costained with DAPI, anti-α smooth muscle actin, or biotinylated Ulex Europaeus Agglutinin I to identify nuclei, pericytes, and endothelial cells, respectively. Whole tissue and purified single cell populations underwent polymerase chain reaction (PCR) for FSHR. Positive control specimens (ovary, sertoli cells) and negative control tissues (skin/subcutis, hepatic cells) were included.
Immunofluorescence of 9 IHs demonstrated that FSHR was enriched in pericytes compared with endothelial cells. Follicle-stimulating hormone receptor was expressed in 6 of 6 whole tissue IHs along with the positive control via PCR. Follicle-stimulating hormone receptor was not present in the negative control samples. Four of 5 sets of pericytes expressed FSHR by PCR. Neither IH endothelial cells, IH stem cells, nor negative control cells exhibited FSHR by PCR.
Because the secretion of FSH correlates with the growth pattern of IH, FSH might be involved in the disease process. Follicle-stimulating hormone receptor is enriched in the pericytes of IH, suggesting that this cell type may be involved in the pathogenesis of the tumor.
婴儿血管瘤（IH）的生命周期与促卵泡激素（FSH）的分泌相同。我们之前已经表明，婴儿血管瘤含有促卵泡激素受体（FSHR）。本研究的目的是确定婴儿血管瘤中哪种细胞类型表达促卵泡激素受体。
使用在临床指示的外科手术过程中获取的人类增殖期婴儿血管瘤组织。石蜡切片和分离的细胞群体（内皮细胞、周细胞、干细胞）进行促卵泡激素受体免疫荧光检测。组织分别用4′,6-二脒基-2-苯基吲哚（DAPI）、抗α平滑肌肌动蛋白或生物素化荆豆凝集素I进行复染，以分别识别细胞核、周细胞和内皮细胞。对整个组织和纯化的单细胞群体进行促卵泡激素受体的聚合酶链反应（PCR）。纳入阳性对照标本（卵巢、支持细胞）和阴性对照组织（皮肤/皮下组织、肝细胞）。
9个婴儿血管瘤的免疫荧光显示，与内皮细胞相比，周细胞中促卵泡激素受体更为丰富。通过PCR检测，6个婴儿血管瘤全组织标本中有6个与阳性对照一样表达促卵泡激素受体。阴性对照样本中未检测到促卵泡激素受体。5组周细胞中有4组通过PCR检测到促卵泡激素受体表达。婴儿血管瘤内皮细胞、婴儿血管瘤干细胞及阴性对照细胞经PCR检测均未显示促卵泡激素受体。
由于促卵泡激素的分泌与婴儿血管瘤的生长模式相关，促卵泡激素可能参与了疾病过程。促卵泡激素受体在婴儿血管瘤的周细胞中更为丰富，提示该细胞类型可能参与了肿瘤的发病机制。
