Department of Pharmacology, Faculty of Pharmacy, University of Debrecen, 4012 Debrecen, Hungary.
Department of Animal Husbandry, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agriculture, Food Science and Environmental Management, University of Debrecen, 4012 Debrecen, Hungary.
Int J Mol Sci. 2018 Mar 28;19(4):1017. doi: 10.3390/ijms19041017.
Recent evidence from studies suggests that aged black garlic also has an effect on health. The major aim of the present study is to compare the effect of raw and aged black garlic on postischemic cardiac recovery. Male Sprague Dawley rats were randomly divided into three groups. Animals of the first group were fed with raw garlic, animals of the second group received aged black garlic, while the third group served as vehicle-treated controls. Upon conclusion of the treatment, isolated hearts were undertaken to ischemia/reperfusion. Heart function and infarct size were measured and the level of HO-1 and iNOS were studied. Superior postischemic cardiac function and reduced infarct size in both garlic treated groups compared to the drug-free control group, indicated cardioprotective effects. However, no significant differences between the garlic treated groups were observed. Western blot analysis revealed that raw garlic enhanced the level of HO-1 before ischemia, while in ischemic samples, we found elevated HO-1 expression in both garlic treated groups. The level of iNOS was the same before ischemia in all groups, however, a markedly reduced iNOS level in ischemic/reperfused hearts originating from control and raw garlic treated animals was observed. Samples from aged black garlic treated animals demonstrated that the level of iNOS was not significantly reduced after ischemia/reperfusion. Taken together these results indicate that not only raw but also aged black garlic possess a cardioprotective effect.
最近的研究证据表明，陈化黑蒜对健康也有影响。本研究的主要目的是比较生黑蒜和陈化黑蒜对缺血后心脏恢复的影响。雄性 Sprague Dawley 大鼠随机分为三组。第一组动物喂食生蒜，第二组动物给予陈化黑蒜，第三组作为药物对照组。治疗结束后，进行离体心脏缺血/再灌注。测量心功能和梗死面积，并研究 HO-1 和 iNOS 的水平。与无药物对照组相比，大蒜处理组的缺血后心脏功能明显改善，梗死面积减小，表明具有心脏保护作用。然而，大蒜处理组之间没有观察到显著差异。Western blot 分析显示，生蒜在缺血前增强了 HO-1 的水平，而在缺血样本中，我们发现大蒜处理组的 HO-1 表达均升高。各组缺血前 iNOS 水平相同，但在缺血/再灌注心脏中，对照组和生蒜处理组的 iNOS 水平明显降低。来自陈化黑蒜处理组的样本表明，缺血/再灌注后 iNOS 水平没有明显降低。综上所述，这些结果表明，不仅生黑蒜，而且陈化黑蒜都具有心脏保护作用。
