Langmuir. 2018 Apr 24;34(16):4750-4761. doi: 10.1021/acs.langmuir.8b00535. Epub 2018 Apr 11.
A siloxane surface-anchored quaternary ammonium salt (AQAS: BIOSAFE HM4100 in this study) has been chemisorbed onto a quartz substrate. The aim of this study is to elucidate, using atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS), the structure of the chemisorbed AQAS layers. The AQAS biocide includes a C alkyl chain previously invoked in lysis potency. The AQAS coverage appears in zones on the surface, which include a first layer (2.6 ± 0.1 nm) and multilayering that were explored using AFM. The XPS data exhibited two N 1s signals at about 402 and 399 eV, with only the former exhibiting angular dependence. This signal at 402 eV was assigned to the first anchored layer with perpendicular orientation determined by the AQAS anchoring to the surface. In preliminary AFM studies of bacteria on these AQAS surfaces, perturbations on the Staphylococcus aureus cells and the degradation of Escherichia coli cells suggest lysis potency.
一种硅烷表面锚定的季铵盐（AQAS：本研究中的 BIOSAFE HM4100）已被化学吸附到石英基底上。本研究的目的是使用原子力显微镜（AFM）和 X 射线光电子能谱（XPS）阐明化学吸附的 AQAS 层的结构。这种季铵盐杀生剂包括先前在溶胞活力中提到的 C 烷基链。AQAS 覆盖层出现在表面的区域上，其中包括第一层（2.6 ± 0.1nm）和多层，这些层使用 AFM 进行了探索。XPS 数据显示了两个 N 1s 信号，约为 402 和 399eV，只有前一个信号表现出角度依赖性。该 402eV 的信号被分配给第一个垂直定向的锚定层，这是由 AQAS 对表面的锚固决定的。在对这些 AQAS 表面上细菌的初步 AFM 研究中，金黄色葡萄球菌细胞的扰动和大肠杆菌细胞的降解表明了溶胞活力。
