Department of Molecular Medicine and Pathology, Waipapa Taumata Rau University of Auckland, Auckland, New Zealand.
NZ Product Accelerator, Faculty of Engineering, Waipapa Taumata Rau University of Auckland, Auckland, New Zealand.
J Appl Microbiol. 2022 Oct;133(4):2583-2598. doi: 10.1111/jam.15729. Epub 2022 Jul 31.
To determine the antimicrobial potency of a surface-anchored quaternary ammonium salt (SAQAS)-based biocide during in vitro wet and dry fomite assays and to determine the mechanism of killing bacteria on the surface.
Wet and dry fomite assays were established in vitro for a commercially available biocide (SAQAS-A) applied to glass and low-density polyethylene (LDPE) surfaces. Both wet and dry fomite tests showed the active killing of Gram-positive and Gram-negative bacteria but not endospores. Assays measuring membrane permeability (ATP and DNA release), bacterial membrane potential and bacterial ROS production were correlated with the time-to-kill profiles to show SAQAS-A activity in suspension and applied to a surface.
SAQAS-A is an effective biocide against model strains of vegetative bacteria. The killing mechanism for SAQAS-A observed minimal membrane depolarization, a surge in ROS production and assessment of membrane permeability supported the puncture of cells in both suspension and surface attachment, leading to cell death.
SAQAS represents effective surface biocides against single challenges with bacteria through a mechanical killing ability that supports real-world application if their durability can be demonstrated to maintain residual activity.
在体外湿和干污染物检测中确定基于季铵盐（SAQAS）的表面锚定杀生物剂的抗菌效力，并确定表面杀菌的机制。
在体外为一种市售杀生物剂（SAQAS-A）建立了湿和干污染物检测，应用于玻璃和低密度聚乙烯（LDPE）表面。湿和干污染物检测均显示出对革兰氏阳性和革兰氏阴性细菌的有效杀灭作用，但对内生孢子无效。测量膜通透性（ATP 和 DNA 释放）、细菌膜电位和细菌 ROS 产生的检测与杀菌时间曲线相关，以显示 SAQAS-A 在悬浮液中和应用于表面的活性。
SAQAS-A 是一种有效的杀菌剂，可有效杀灭模式菌株的营养细菌。观察到 SAQAS-A 的杀菌机制是最小的膜去极化，ROS 产生的激增以及膜通透性的评估支持了在悬浮液和表面附着中细胞的穿孔，导致细胞死亡。
SAQAS 是一种有效的表面杀生物剂，可有效应对细菌的单一挑战，具有机械杀菌能力，如果能够证明其耐用性能够保持残留活性，则支持实际应用。
