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NS5与含有西尼罗河病毒特异性聚合酶活性的细胞组分的解离。

Dissociation of NS5 from cell fractions containing West Nile virus-specific polymerase activity.

作者信息

Grun J B, Brinton M A

机构信息

Wistar Institute, Philadelphia, Pennsylvania 19104-4268.

出版信息

J Virol. 1987 Nov;61(11):3641-4. doi: 10.1128/JVI.61.11.3641-3644.1987.

DOI:10.1128/JVI.61.11.3641-3644.1987
PMID:2959795
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255969/
Abstract

West Nile virus replication complexes were partially purified from cytoplasmic extracts of virus-infected cells by centrifugation through a 20% glycerol cushion. Numerous cell proteins, as well as the largest nonstructural protein, NS5, were separated from the replication complexes without significant loss of in vitro West Nile virus polymerase activity.

摘要

通过在20%甘油垫层上离心，从病毒感染细胞的细胞质提取物中部分纯化了西尼罗河病毒复制复合体。许多细胞蛋白以及最大的非结构蛋白NS5与复制复合体分离，而体外西尼罗河病毒聚合酶活性没有显著损失。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8df/255969/2853008fea76/jvirol00102-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8df/255969/2853008fea76/jvirol00102-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8df/255969/2853008fea76/jvirol00102-0304-a.jpg

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