Lee Katherine T W, Gopalan Vinod, Lam Alfred K
Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia.
Methods Mol Biol. 2018;1756:195-212. doi: 10.1007/978-1-4939-7734-5_18.
Somatic copy-number alterations are commonly found in cancer and play key roles in activating oncogenes and deactivating tumor suppressor genes. Digital polymerase chain reaction is an effective way to detect the changes in copy number. In esophageal adenocarcinoma, detection of somatic copy-number alterations could predict the prognosis of patients as well as the response to therapy. This chapter will review the methods involved in digital polymerase chain reaction for the research or potential clinical applications in esophageal adenocarcinoma.
体细胞拷贝数改变在癌症中普遍存在，在激活癌基因和使肿瘤抑制基因失活方面发挥关键作用。数字聚合酶链反应是检测拷贝数变化的有效方法。在食管腺癌中，检测体细胞拷贝数改变可以预测患者的预后以及对治疗的反应。本章将综述数字聚合酶链反应在食管腺癌研究或潜在临床应用中所涉及的方法。
