Pillai Suja, Gopalan Vinod, Lam Alfred K
Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia.
School of Biomedical Sciences, Faculty of Medicine, University of Queensland, Brisbane, Australia.
Methods Mol Biol. 2018;1756:231-246. doi: 10.1007/978-1-4939-7734-5_20.
Next-generation sequencing refers to the high-throughput DNA sequencing technologies, which are capable of sequencing large numbers of different DNA sequences in a single/parallel reaction. It is a powerful tool to identify inherited and acquired genetic alterations associated with the development of esophageal adenocarcinoma. Whole-genome sequencing is the most comprehensive but expensive, whereas whole-exome sequencing is cost-effective but it only works for the known genes. Thus, second-generation sequencing methods can provide a complete picture of the esophageal adenocarcinoma genome by detecting and discovering different type of alterations in the cancer. This would help in diagnostics and will further help in developing personalized medicine in esophageal adenocarcinoma.
下一代测序是指高通量DNA测序技术，这些技术能够在单个/平行反应中对大量不同的DNA序列进行测序。它是识别与食管腺癌发生相关的遗传和后天遗传改变的有力工具。全基因组测序是最全面但成本高昂的，而全外显子组测序具有成本效益，但仅适用于已知基因。因此，第二代测序方法可以通过检测和发现癌症中的不同类型改变，提供食管腺癌基因组的全貌。这将有助于诊断，并进一步有助于开发食管腺癌的个性化药物。
