Paquette Thierry, Leblond Hugues, Piché Mathieu
Department of Chiropractic, Université du Québec à Trois-Rivières, 3351 Boul. Des Forges, C.P. 500, Trois-Rivières, QC, Canada, G9A 5H7.
CogNAC Research Group, Université du Québec à Trois-Rivières, 3351 Boul. Des Forges, C.P. 500, Trois-Rivières, QC, Canada, G9A 5H7.
J Physiol Sci. 2019 Jan;69(1):13-21. doi: 10.1007/s12576-018-0607-7. Epub 2018 Mar 29.
Neurological examination remains the primary clinical investigation in patients with spinal cord injury. However, neuroimaging methods such as functional magnetic resonance imaging (fMRI) are promising tools for following functional changes in the course of injury, disease and rehabilitation. However, the relationship between neuronal activity and blood flow in the spinal cord on which fMRI relies has been largely overlooked. The objective of this study was to examine neurovascular coupling in the spinal cord of decerebrated rats during electrical stimulation of the sciatic nerve with and without isoflurane anesthesia (1.2%). Local field potentials (LFP) and spinal cord blood flow (SCBF) were recorded simultaneously in the lumbosacral enlargement. Isoflurane did not significantly alter LFP (p = 0.53) and SCBF (p = 0.57) amplitude. Accordingly, neurovascular coupling remained comparable with or without isoflurane anesthesia (p = 0.39). These results support the use of isoflurane in rodents to investigate nociceptive functions of the spinal cord using fMRI.
神经系统检查仍然是脊髓损伤患者的主要临床检查方法。然而，诸如功能磁共振成像（fMRI）等神经成像方法是追踪损伤、疾病及康复过程中功能变化的有前景的工具。然而，fMRI所依赖的脊髓中神经元活动与血流之间的关系在很大程度上被忽视了。本研究的目的是在有无1.2%异氟烷麻醉的情况下，电刺激去大脑大鼠的坐骨神经时，检查其脊髓中的神经血管耦合。在腰骶膨大处同时记录局部场电位（LFP）和脊髓血流（SCBF）。异氟烷并未显著改变LFP（p = 0.53）和SCBF（p = 0.57）的幅度。因此，有无异氟烷麻醉时神经血管耦合相当（p = 0.39）。这些结果支持在啮齿动物中使用异氟烷，通过fMRI来研究脊髓的伤害感受功能。
