Zhao Yu-Jing, Li Jian-Kuan, Zhang Xin, Gao Jian-Ping
College of Pharmacy, Shanxi Medical University, Taiyuan 030001, China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(4):760-765. doi: 10.19540/j.cnki.cjcmm.20180104.013.
The present study is to study the chemical constituents from ethanol extract of Psidium guajava leaves. The constituents were separated and purified by silica gel column chromaiographios over, macroporous resin D-101, Sephadex LH-20, and ODS. Six flavonoids compounds were isolated and identified as quercetin(1), quercetin-3---D-arabinopyranoside(2), quercetin-3---D-ribopyranoside(3), quercetin-3---D-galactopyranoside(4), quercetin-3---D-glucopyranoside(5), and quercetin-3--D-xylpyranoside(6). The antioxidant effects of six flavonoids was evaluated by scavenging ability of DPPH, superoxide anion, ABTS·⁺, and reducing effect of Fe³⁺ as well as total antioxidant capacity(FRAP). Vitamin C was used as positive control. The results indicated that six flavonoids exhibited significant antioxidant effects.
本研究旨在研究番石榴叶乙醇提取物中的化学成分。通过硅胶柱色谱、大孔树脂D - 101、葡聚糖凝胶LH - 20和十八烷基硅烷键合硅胶（ODS）对其成分进行分离和纯化。分离鉴定出6种黄酮类化合物，分别为槲皮素（1）、槲皮素 - 3 - β - D - 阿拉伯吡喃糖苷（2）、槲皮素 - 3 - β - D - 核糖吡喃糖苷（3）、槲皮素 - 3 - β - D - 半乳糖吡喃糖苷（4）、槲皮素 - 3 - β - D - 葡萄糖吡喃糖苷（5）和槲皮素 - 3 - β - D - 木糖吡喃糖苷（6）。通过DPPH、超氧阴离子、ABTS·⁺清除能力、Fe³⁺还原能力以及总抗氧化能力（FRAP）对6种黄酮类化合物的抗氧化作用进行评价。维生素C用作阳性对照。结果表明，6种黄酮类化合物均表现出显著的抗氧化作用。
