School of Biology and Biological Engineering, South China University of Technology, Guangzhou, P. R. China.
Jiangmen Nanyue Guava Farmer Cooperatives, Jiangmen, P. R. China.
J Sep Sci. 2017 Oct;40(19):3817-3829. doi: 10.1002/jssc.201700477. Epub 2017 Aug 31.
Flavonoids are the main active components in Psidium guajava leaves and have many multi-physiological functions. In this study, the flavonoid compositions were identified in the Psidium guajava leaves samples using a high-performance liquid chromatography with time-of-flight electrospray ionization mass spectrometry method. A high-performance liquid chromatography fingerprint method, combined with chemometrics, was used to perform a quality assessment of the Psidium guajava leaves samples. The eight identified flavonoid compounds including rutin, isoquercitrin, quercetin-3-O-β-d-xylopyranoside, quercetin-3-O-α-l-arabinopyranoside, avicularin, quercitrin, quercetin, and kaempferol were used as the chemical markers. The antioxidant activity of 15 batches of samples was examined using three different methods, and the results revealed the Psidium guajava leaves samples that had higher contents of the flavonoid compounds, glycoside and aglycone, possessed the highest antioxidant capacities. Consequently, a combination of chromatographic fingerprints and chemometric analyses was used for a quality assessment of Psidium guajava leaf tea and its derived products, which can lay the foundation for the development of plant tea resources or other herbs.
类黄酮是番石榴叶的主要活性成分，具有多种生理功能。本研究采用高效液相色谱-飞行时间电喷雾离子质谱法鉴定了番石榴叶样品中的类黄酮成分。采用高效液相色谱指纹图谱法结合化学计量学方法对番石榴叶样品进行了质量评估。鉴定出的 8 种类黄酮化合物包括芦丁、异槲皮苷、槲皮素-3-O-β-吡喃葡萄糖苷、槲皮素-3-O-α-吡喃阿拉伯糖苷、鸡纳苷、根皮苷、槲皮素和山柰酚，这些化合物被用作化学标志物。采用三种不同方法检测了 15 批样品的抗氧化活性，结果表明，类黄酮化合物、糖苷和苷元含量较高的番石榴叶样品具有较高的抗氧化能力。因此，采用色谱指纹图谱和化学计量学分析相结合的方法对番石榴叶茶及其衍生产品进行质量评估，可以为开发植物茶资源或其他草药奠定基础。
