Key Laboratory of Chemical Synthesis and Pollution Control of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, China.
School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 5;238:118405. doi: 10.1016/j.saa.2020.118405. Epub 2020 May 5.
A new fluorescent probe Lyso-Fl has been facilely prepared by an esterification reaction of spironolactone fluoran dye Rdi with ethanol, which shows viscosity-selective response by fluorescence. The new probe delivers obvious fluorescence signal enhancement when environmental viscosity changes from 1.01 cP (water) to 1256 cP (98% glycerol). And, both the emission intensity (575 nm) and fluorescence lifetime of Lyso-Fl exhibit individually good linear relationships with the solution viscosity. Besides, Lyso-Fl gives a selective response to viscosity among various biological species and exhibits pH-independent (1-10) fluorescent signals towards viscosity. More importantly, Lyso-Fl shows low cytotoxicity and can be utilized for monitoring of dexamethasone-stimulated viscosity enhancement by cell imaging with excellent lysosome-targeted performance, promoting it a promising fluorescent probe for lysosomal viscosity detection.
一种新的荧光探针 Lyso-Fl 通过螺内酯氟蒽染料 Rdi 与乙醇的酯化反应简便地制备，该探针在荧光方面表现出对粘度的选择性响应。当环境粘度从 1.01 cP（水）变化到 1256 cP（98%甘油）时，新探针会产生明显的荧光信号增强。并且，Lyso-Fl 的发射强度（575nm）和荧光寿命都与溶液粘度呈现出良好的线性关系。此外，Lyso-Fl 在各种生物物质中对粘度表现出选择性响应，并且对粘度表现出 pH 独立（1-10）的荧光信号。更重要的是，Lyso-Fl 表现出低细胞毒性，并且可以通过细胞成像用于监测地塞米松刺激的粘度增强，具有优异的溶酶体靶向性能，因此有望成为溶酶体粘度检测的荧光探针。
