Optical Materials Engineering Laboratory , ETH Zurich , Zurich 8092 , Switzerland.
ACS Appl Mater Interfaces. 2018 Apr 18;10(15):12497-12503. doi: 10.1021/acsami.8b02225. Epub 2018 Apr 9.
Graphene oxide (GO) has attracted significant interest as a template material for multiple applications due to its two-dimensional nature and established functionalization chemistries. However, for applications toward stem cell culture and differentiation, GO is often reduced to form reduced graphene oxide, resulting in a loss of oxygen content. Here, we induce a phase transformation in GO and demonstrate its benefits for enhanced stem cell culture and differentiation while conserving the oxygen content. The transformation results in the clustering of oxygen atoms on the GO surface, which greatly improves its ability toward substance adherence and results in enhanced differentiation of human mesenchymal stem cells toward the osteogenic lineage. Moreover, the conjugating ability of modified GO strengthened, which was examined by auxiliary osteogenic growth peptide conjugation. Overall, our work demonstrates GO's potential for stem cell applications while maintaining its oxygen content, which could enable further functionalization and fabrication of novel nano-biointerfaces.
氧化石墨烯（GO）由于其二维性质和成熟的功能化化学，作为模板材料在多种应用中引起了极大的兴趣。然而，对于干细胞培养和分化的应用，GO 通常被还原为还原氧化石墨烯，导致氧含量的损失。在这里，我们诱导 GO 的相转变，并证明其在保持氧含量的同时，增强干细胞培养和分化的益处。该转变导致 GO 表面上的氧原子聚集，极大地提高了其对物质的附着能力，并促进了人骨髓间充质干细胞向成骨系的分化。此外，通过辅助成骨生长肽的偶联，证实了修饰后的 GO 的偶联能力增强。总的来说，我们的工作证明了 GO 在保持其氧含量的情况下在干细胞应用中的潜力，这可能使进一步的功能化和新型纳米生物界面的制造成为可能。
