Oh Jun-Sung, Park Jeong-Sun, Lee Eun-Jung
Department of Nano-biomedical Science, BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Korea.
Biomedicines. 2021 May 2;9(5):501. doi: 10.3390/biomedicines9050501.
Statin derivatives traditionally have been used for the treatment of hyperlipidemia, but recent studies have shown their ability to regulate bone metabolism and promote bone growth. In this study, simvastatin (Sim), a new therapeutic candidate for bone regeneration, was combined with graphene oxide (GO), which has recently attracted much interest as a drug delivery method, to produce a compound substance effective for bone regeneration. To create a stable and homogenous complex with Sim, GO was modified with polyethylenimine, and the effect of modification was analyzed using Fourier transform infrared spectroscopy, zeta potential, and cytotoxicity testing. More specifically, the osteogenic differentiation potential expected by the combination of the two effective materials for osteogenic differentiation, GO and Sim, was evaluated in mesenchymal stem cells. Compared with control groups with GO and Sim used separately, the GO/Sim complex showed excellent osteogenic differentiation properties, with especially enhanced effects in the complex containing < 1 μM Sim.
他汀类衍生物传统上用于治疗高脂血症，但最近的研究表明它们具有调节骨代谢和促进骨生长的能力。在本研究中，辛伐他汀（Sim）作为一种用于骨再生的新型治疗候选药物，与氧化石墨烯（GO）相结合，氧化石墨烯作为一种药物递送方法最近引起了广泛关注，以制备对骨再生有效的复合物质。为了与Sim形成稳定且均匀的复合物，用聚乙烯亚胺对GO进行修饰，并使用傅里叶变换红外光谱、zeta电位和细胞毒性测试分析修饰效果。更具体地说，在间充质干细胞中评估了两种对成骨分化有效的材料GO和Sim组合所预期的成骨分化潜能。与单独使用GO和Sim的对照组相比，GO/Sim复合物表现出优异的成骨分化特性，在含有<1μM Sim的复合物中效果尤其增强。
