Schütten H J, Johannessen A C, Torp-Pedersen C, Sander-Jensen K, Bie P, Warberg J
Department of Medical Physiology, University of Copenhagen, Denmark.
Acta Physiol Scand. 1987 Oct;131(2):265-72. doi: 10.1111/j.1748-1716.1987.tb08236.x.
A sensitive radio-immunoassay (RIA) for the measurement of human alpha-atrial natriuretic peptide (ANP) in extracted plasma was developed and used in a study of the possible effect of posture on the concentration of ANP in plasma. The least detectable quantity was less than 2 pg per tube equivalent to 5 pg ml-1 plasma. In the middle sensitivity range (approximately 50 pg per tube), the within-assay and between-assay coefficients of variation were 4.0 and 2.8%, respectively. The recovery of ANP added to plasma prior to extraction was 95-101%. High pressure liquid chromatography (HPLC) of plasma extracts revealed that endogenous ANP was eluted in the same fractions as synthetic ANP. In order to investigate the effect of posture on the concentration of ANP in plasma six healthy volunteers were exposed to five positions in the following sequence: supine, standing, sitting, supine and 10 degrees head-down tilt on a tilt-table. The concentration of ANP was lower in the standing and sitting position than in the supine and head-down tilted position. In another study six healthy volunteers were subjected to passive tilting on a tilt-table in order to evaluate the effect of tilting on blood pressure (BP), heart rate, central venous pressure (CVP) and the concentration of ANP in plasma. It was found that a fall in CVP was accompanied by a significant decrease in the concentration of ANP and that a rise in CVP was accompanied by a rapid increase in the concentration of ANP in plasma. The results are in agreement with the hypothesis that CVP is a physiological stimulus for the secretion of ANP.
我们开发了一种用于测量提取血浆中人类α-心房利钠肽（ANP）的灵敏放射免疫分析（RIA）方法，并将其用于一项关于姿势对血浆中ANP浓度可能影响的研究。最低检测量小于每管2皮克，相当于血浆中5皮克/毫升。在中等灵敏度范围内（每管约50皮克），批内和批间变异系数分别为4.0%和2.8%。提取前添加到血浆中的ANP回收率为95 - 101%。血浆提取物的高压液相色谱（HPLC）显示，内源性ANP与合成ANP在相同馏分中洗脱。为了研究姿势对血浆中ANP浓度的影响，六名健康志愿者按以下顺序处于五个体位：仰卧位、站立位、坐位、仰卧位以及在倾斜台上头向下倾斜10度。站立位和坐位时ANP的浓度低于仰卧位和头向下倾斜位。在另一项研究中，六名健康志愿者在倾斜台上进行被动倾斜，以评估倾斜对血压（BP）、心率、中心静脉压（CVP）和血浆中ANP浓度的影响。结果发现，CVP下降伴随着ANP浓度显著降低，而CVP升高伴随着血浆中ANP浓度迅速增加。这些结果与CVP是ANP分泌的生理刺激这一假设相符。
