Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan.
MagQu Co., Ltd., New Taipei 231, Taiwan.
Sensors (Basel). 2018 Mar 30;18(4):1043. doi: 10.3390/s18041043.
An alternating-current magnetosusceptometer of antibody-functionalized magnetic nanoparticles (MNPs) was developed for immunomagnetic reduction (IMR). A high-sensitivity, high-critical-temperature superconducting quantum interference device was used in the magnetosusceptometer. Minute levels of biomarkers of early-stage neurodegeneration diseases were detectable in serum, but measuring each biomarker required approximately 4 h. Hence, an eight-channel platform was developed in this study to fit minimal screening requirements for Alzheimer's disease. Two consistent results were measured for three biomarkers, namely Aβ40, Aβ42, and tau protein, per human specimen. This paper presents the instrument configuration as well as critical characteristics, such as the low noise level variations among channels, a high signal-to-noise ratio, and the coefficient of variation for the biomarkers' IMR values. The instrument's ultrahigh sensitivity levels for the three biomarkers and the substantially shorter total measurement time in comparison with the previous single- and four-channels platforms were also demonstrated in this study. Thus, the eight-channel instrument may serve as a powerful tool for clinical high-throughput screening of Alzheimer's disease.
一种用于免疫磁还原（IMR）的抗体功能化磁性纳米粒子（MNP）的交流磁强计已经被开发出来。磁强计中使用了高灵敏度、高临界温度超导量子干涉装置。在血清中可以检测到早期神经退行性疾病生物标志物的微量水平，但测量每个生物标志物大约需要 4 小时。因此，本研究开发了一个 8 通道平台，以满足阿尔茨海默病最小筛查要求。每个人体标本可同时测量三种生物标志物 Aβ40、Aβ42 和 tau 蛋白的两个一致结果。本文介绍了仪器的配置以及关键特性，例如通道之间的低噪声电平变化、高信噪比以及生物标志物 IMR 值的变异系数。与之前的单通道和四通道平台相比，该仪器对三种生物标志物具有超高灵敏度水平，并且总测量时间大大缩短，这一点在本研究中也得到了证明。因此，八通道仪器可以作为一种强大的工具，用于临床高通量筛选阿尔茨海默病。
