Allergy Department, Beijing Children's hospital, Capital Medical University, Beijing, China, No.56 Nanlishi Road, Xicheng District, 100045, Beijing P.R.China.
Asian Pac J Allergy Immunol. 2019 Jun;37(2):65-72. doi: 10.12932/AP-140317-0047.
To investigate whether there were differences in Th17 cells mediated immunological responses among asthmatics with or without allergic rhinitis.
A case-control comparison was conducted in a cohort of 67 children with asthma (AS), 50 children with allergic rhinitis (AR), 52 children with both AS and AR (ASR), 25 infectious rhinitis (IR), and 55 healthy controls (HC). The percentages of circulating Th17 cells were determined by flow cytometry. The Th2- and Th17-related cytokines in plasma and culture supernatants were measured by enzyme-linked immunosorbent assay. The effect of proinflammation cytokine IL-17E on Th2 cytokines production from human T helper (Th) lymphocytes was analyzed.
(1) A inter-group comparison revealed that Th17 cells levels were highest in ASR group [(0.89% ± 0.27) %], following by AS group [(0.82 ± 0.29) %] and AR group[(0.78 ± 0.17) %] (P < 0.05). (2) After in-vitro stimulation with house dust mite (HDM) antigen, the levels of IL-4 and IL-17E in culture supernatants of PBMCs from allergic children (AS group, AR group and ASR group) were significantly enhanced. (3) The release of Th2 cytokines from IL-17E treated Th cells of allergic children (AS group, AR group and ASR group) were significantly induced, no similar result was observed in IR group and HC group. CONCLUSIONonclusion: Our findings preliminarily revealed that Th17 cell and its related cytokines might be involved in pathogenesis of airway inflammation diseases, and also presenting varying immunological characteristics among asthmatic children with or without allergic rhinitis.
探讨过敏性鼻炎合并哮喘患儿与单纯哮喘或过敏性鼻炎患儿之间 Th17 细胞介导的免疫应答是否存在差异。
采用病例对照研究，纳入哮喘患儿 67 例（哮喘组）、过敏性鼻炎患儿 50 例（鼻炎组）、过敏性鼻炎合并哮喘患儿 52 例（哮喘鼻炎组）、感染性鼻炎患儿 25 例（感染组）和健康对照 55 例（健康组）。采用流式细胞术检测外周血 Th17 细胞比例，酶联免疫吸附试验检测血浆和培养上清液中 Th2 和 Th17 相关细胞因子，分析促炎细胞因子 IL-17E 对人辅助性 T 淋巴细胞（Th）细胞 Th2 细胞因子产生的影响。
（1）组间比较显示，哮喘鼻炎组 Th17 细胞比例最高[（0.89±0.27）%]，其次为哮喘组[（0.82±0.29）%]和鼻炎组[（0.78±0.17）%]（P<0.05）。（2）体外经屋尘螨（HDM）抗原刺激后，过敏性儿童（哮喘组、鼻炎组和哮喘鼻炎组）PBMC 培养上清液中 IL-4 和 IL-17E 水平显著升高。（3）IL-17E 处理后过敏性儿童（哮喘组、鼻炎组和哮喘鼻炎组）Th 细胞 Th2 细胞因子释放明显增加，但在感染组和健康对照组未观察到类似结果。
本研究初步表明，Th17 细胞及其相关细胞因子可能参与气道炎症性疾病的发病机制，且在合并或不合并过敏性鼻炎的哮喘患儿中具有不同的免疫学特征。
