Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
Post-Graduate Program in Microbiology, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
Int Arch Allergy Immunol. 2021;182(12):1155-1168. doi: 10.1159/000517478. Epub 2021 Aug 4.
Major depressive disorder (MDD) can impact the severity of allergic rhinitis (AR) and asthma (AA). Here, we evaluated the cytokine production by T-cells from AR and AA patients with or without MDD. The effect of serotonin on the in vitro T-cell response was also evaluated.
The cytokines produced by activated T-cells were measured by Luminex and flow cytometry. In some cell cultures, serotonin was added.
MDD not only enhanced the production of Th2- and Th17-related cytokines, but also, the levels of interleukin (IL)-5 and IL-17 were directly correlated with the severity of depression and anxiety symptoms. As compared with AR, the levels of IL-17 were higher and the release of IL-10 was lower in activated T-cell cultures from AA patients, mainly those with MDD. In AA/MDD patients, the severity of anxiety symptoms and lung disease was directly correlated with Th17-like and hybrid Th2/Th17 cells, but inversely correlated with IL-10-secreting CD4+ T-cells. Finally, the addition of serotonin reduced the production of Th2- and Th17-related cytokines, but elevated IL-10 secretion in cell cultures from both AR and AA patients.
Our findings suggest that not only the occurrence of MDD but also the severity of anxiety symptoms, may adversely affect the outcome of allergic reactions by favoring the production of cytokines implicated in the pathogenesis of AR and AA, a phenomenon that was attenuated by serotonin.
重度抑郁症（MDD）可影响变应性鼻炎（AR）和哮喘（AA）的严重程度。在此，我们评估了伴有或不伴有 MDD 的 AR 和 AA 患者 T 细胞的细胞因子产生情况。还评估了血清素对体外 T 细胞反应的影响。
通过 Luminex 和流式细胞术测量激活的 T 细胞产生的细胞因子。在一些细胞培养物中添加了血清素。
MDD 不仅增强了 Th2 和 Th17 相关细胞因子的产生，而且白细胞介素（IL）-5 和 IL-17 的水平与抑郁和焦虑症状的严重程度直接相关。与 AR 相比，AA 患者激活的 T 细胞培养物中 IL-17 的水平更高，IL-10 的释放更低，尤其是 MDD 患者。在 AA/MDD 患者中，焦虑症状和肺部疾病的严重程度与 Th17 样和混合 Th2/Th17 细胞直接相关，而与分泌 IL-10 的 CD4+T 细胞呈负相关。最后，添加血清素可减少 AR 和 AA 患者培养物中 Th2 和 Th17 相关细胞因子的产生，但可增加 IL-10 的分泌。
我们的研究结果表明，不仅 MDD 的发生，而且焦虑症状的严重程度，都可能通过有利于 AR 和 AA 发病机制中涉及的细胞因子的产生而对过敏反应的结果产生不利影响，而这种现象被血清素所减弱。
