School of Materials Science and Engineering, Zhengzhou University, Zhengzhou, 450002, China.
Orthopaedic Institute of Henan Province, Luoyang, 471000, China.
J Mater Sci Mater Med. 2018 Mar 30;29(4):44. doi: 10.1007/s10856-018-6050-8.
Mg-Zn-Y-Nd-Zr alloy has been developed as a new type of biodegradable orthopaedic implant material by the authors' research group with its excellent mechanical properties and controllable degradation rate. In this study, the cytocompatibility of Mg-Zn-Y-Nd-Zr alloy was systematically evaluated through in vitro cell culture method. MTT assay was applied to evaluate the cytotoxicity of Mg-Zn-Y-Nd-Zr alloy and no toxic effect was observed on L929 and MC3T3-E1 cells followed the protocol of ISO 10993 standard. Considering the potential ion accumulation in the bony environment, this study further investigated the cytotoxic effect of accumulated metallic ions during the alloy degradation by extending the extract preparation time. When the extract preparation time was prolonged to 1440 h, the accumulated metallic ions leaded to severe cell apoptosis, of which the combined ion concentration was determined as 39.5-65.8 µM of Mg, 3.5-5.9 µM of Zn, 0.44-0.74 µM of Y, 0.3-0.52 µM of Nd and 0.11-0.18 µM of Zr for L929, and 65.8-92.2 µM of Mg, 5.9-8.3 µM of Zn, 0.74-1.04 µM of Y, 0.52-0.73 µM of Nd and 0.18-0.25 µM of Zr for MC3T3-E1 cells. Besides the cell viability assessment, high expression of ALP activity and calcified nodules implied that metal elements in Mg-Zn-Y-Nd-Zr alloys can promote the osteogenic differentiation. Hence, excellent cytocompatibility has equipped Mg-Zn-Y-Nd-Zr alloy as a promising candidate for orthopaedic implant application, which can remarkably guide the magnesium-based alloy design and provide scientific evidence for clinical practice in future.
镁锌钇钕锆合金是由作者所在的研究团队开发的一种新型可生物降解的骨科植入材料，具有优异的力学性能和可控制的降解速率。在本研究中，采用体外细胞培养法系统评价了镁锌钇钕锆合金的细胞相容性。通过 MTT 试验评估了镁锌钇钕锆合金的细胞毒性，按照 ISO 10993 标准的方案，在 L929 和 MC3T3-E1 细胞上未观察到毒性作用。考虑到在骨环境中潜在的离子积累，本研究进一步通过延长提取液制备时间来研究合金降解过程中积累的金属离子的细胞毒性效应。当提取液制备时间延长至 1440 h 时，积累的金属离子导致严重的细胞凋亡，其中复合离子浓度确定为 39.5-65.8 μM 的 Mg、3.5-5.9 μM 的 Zn、0.44-0.74 μM 的 Y、0.3-0.52 μM 的 Nd 和 0.11-0.18 μM 的 Zr，用于 L929；65.8-92.2 μM 的 Mg、5.9-8.3 μM 的 Zn、0.74-1.04 μM 的 Y、0.52-0.73 μM 的 Nd 和 0.18-0.25 μM 的 Zr 用于 MC3T3-E1 细胞。除了细胞活力评估外，碱性磷酸酶活性和钙化结节的高表达表明镁锌钇钕锆合金中的金属元素可以促进成骨分化。因此，良好的细胞相容性使镁锌钇钕锆合金成为一种有前途的骨科植入物候选材料，为未来的镁基合金设计提供了科学依据，并为临床实践提供了科学依据。
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