改良碳青霉烯酶检测试验：鉴别产KPC酶和产金属β-内酰胺酶的克雷伯菌属细菌的简单快速方法。
Modified Carba NP Test: Simple and rapid method to differentiate KPC- and MBL-producing Klebsiella species.
作者信息
Kumar Nitin, Singh Varsha A, Beniwal Vikas, Pottathil Shinu
机构信息
M M Institute of Medical Sciences and Research, Mullana, Ambala, Haryana, India.
Maharishi Markandeshwar University, Mullana, Ambala, Haryana, India.
出版信息
J Clin Lab Anal. 2018 Sep;32(7):e22448. doi: 10.1002/jcla.22448. Epub 2018 Mar 30.
Abstract
BACKGROUND
The aim of this study was to evaluate the modified Carba NP test to differentiate KPC (Klebsiella pneumoniae carbapenemase)- and MBL (metallo-β-lactamase)-producing Klebsiella species.
METHODS
A total of 508 non-duplicate clinical isolates of Klebsiella spp. were processed by modified Carba NP and combined disc tests which were further confirmed by conventional polymerase chain reaction (PCR), a gold standard method for statistical analysis.
RESULTS
Modified Carba NP test demonstrated 91.7% sensitivity, 100% specificity, 100% positive predictive value (PPV) and 99.8% negative predictive value (NPV) for KPC and 96.7%, 100%, 100%, and 99.5% for MBL detection, respectively.
CONCLUSION
The performance of modified Carba NP test was significantly better than combined disc test, fulfilling the requirement of simple and rapid test for clinical applications.
摘要
背景
本研究的目的是评估改良Carba NP试验，以区分产肺炎克雷伯菌碳青霉烯酶（KPC）和产金属β-内酰胺酶（MBL）的克雷伯菌属。
方法
共对508株非重复的克雷伯菌临床分离株进行改良Carba NP试验和复合纸片法检测，并通过传统聚合酶链反应（PCR）进一步确认，PCR是用于统计分析的金标准方法。
结果
改良Carba NP试验检测KPC的灵敏度为91.7%，特异性为100%，阳性预测值（PPV）为100%，阴性预测值（NPV）为99.8%；检测MBL的灵敏度、特异性、PPV和NPV分别为96.7%、100%、100%和99.5%。
结论
改良Carba NP试验的性能明显优于复合纸片法检测，满足临床应用中简单快速检测的要求。
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