Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran.
Biosens Bioelectron. 2018 Jul 1;110:97-102. doi: 10.1016/j.bios.2018.03.003. Epub 2018 Mar 6.
A novel electrochemical immunosensor based on carbon paste electrode (CPE) composed of ionic liquid (IL) and graphite was constructed. It demonstrated good efficiency for quick (each test in 30 s) determination of thyroid stimulating hormone (TSH). Electrode surface was modified by gold nanoparticles in order to immobilize of the thyroid stimulating hormone antibody (anti-TSH) on the CPE. The immunoassay structure was established by sandwiching the antigen (TSH) between the thyroid stimulating hormone antibody on the CPE surface modified with gold nanoparticles and the secondary antibody, polyclonal anti-human-TSH labeled with horseradish peroxidase (HRP-labeled anti-TSH). The signal of differential pulse voltammetry (DPV) was used as a basis for the determination of TSH concentration. This signal is generated by the reaction between O-aminophenol (OAP) and HO catalyzed by HRP. The proposed immunosensor is able to measure the concentration of TSH in a linear range between 0.2 and 90.0 ng/mL with a detection limit 0.1 ± 0.02 ng/mL. In addition, high sensitivity and acceptable stability were achieved by this immunosensor which is promising in the clinical assay of TSH.
基于由离子液体 (IL) 和石墨组成的碳糊电极 (CPE)，构建了一种新型电化学免疫传感器。它在快速测定促甲状腺激素 (TSH) 方面表现出了良好的效果（每次测试用时 30s）。通过金纳米粒子修饰电极表面，将甲状腺刺激素抗体 (anti-TSH) 固定在 CPE 上。免疫分析结构通过将抗原 (TSH) 夹在经金纳米粒子修饰的 CPE 表面上的甲状腺刺激素抗体和标记辣根过氧化物酶的多克隆抗人-TSH（HRP-labeled anti-TSH）之间来建立。差分脉冲伏安法 (DPV) 的信号用作测定 TSH 浓度的基础。该信号是由 HRP 催化的 O-氨基酚 (OAP) 和 HO 之间的反应产生的。所提出的免疫传感器能够在 0.2 到 90.0ng/mL 的线性范围内测量 TSH 的浓度，检测限为 0.1±0.02ng/mL。此外，该免疫传感器具有较高的灵敏度和可接受的稳定性，有望用于 TSH 的临床测定。
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