Choi Jin-Ha, Lee Jin-Ho, Choi Jeong-Woo
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04107, Korea.
School of Biomedical Convergence Engineering, Pusan National University, Yangsan 50612, Korea.
Materials (Basel). 2020 Aug 21;13(17):3677. doi: 10.3390/ma13173677.
Recently, extracellular vesicles (EVs) and their contents have been revealed to play crucial roles in the intrinsic intercellular communications and have received extensive attention as next-generation biomarkers for diagnosis of diseases such as cancers. However, due to the structural nature of the EVs, the precise isolation and characterization are extremely challenging. To this end, tremendous efforts have been made to develop bionano sensors for the precise and sensitive characterization of EVs from a complex biologic fluid. In this review, we will provide a detailed discussion of recently developed bionano sensors in which EVs analysis applications were achieved, typically in optical and electrochemical methods. We believe that the topics discussed in this review will be useful to provide a concise guideline in the development of bionano sensors for EVs monitoring in the future. The development of a novel strategy to monitor various bio/chemical materials from EVs will provide promising information to understand cellular activities in a more precise manner and accelerates research on both cancer and cell-based therapy.
最近，细胞外囊泡（EVs）及其内含物已被揭示在细胞间固有通讯中发挥关键作用，并作为癌症等疾病诊断的下一代生物标志物受到广泛关注。然而，由于EVs的结构特性，其精确分离和表征极具挑战性。为此，人们付出了巨大努力来开发用于从复杂生物流体中精确、灵敏地表征EVs的生物纳米传感器。在这篇综述中，我们将详细讨论最近开发的生物纳米传感器，这些传感器实现了EVs分析应用，典型的有光学和电化学方法。我们相信，本综述中讨论的主题将有助于为未来开发用于监测EVs的生物纳米传感器提供简明指南。开发一种监测来自EVs的各种生物/化学物质的新策略，将为更精确地理解细胞活动提供有前景的信息，并加速癌症和基于细胞的治疗研究。
