State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China.
Bioresour Technol. 2018 Jul;260:9-15. doi: 10.1016/j.biortech.2018.03.070. Epub 2018 Mar 15.
The aim of the present study is to develop an effective production process for α, ω-dodecanedioic acid (DC) biosynthesis using n-dodecane and hydrolysate of Candida cells as substrates by membrane integrated repeated batch fermentation. Cells and n-dodecane were simultaneously recycled during the filtration of fermentation broth (FB) with a 150 kDa ceramic membrane under a cross-flow velocity of 4 m/s and a trans-membrane pressure of 0.2 MPa, and it was also revealed that the cells in the broth could alleviate the membrane fouling during the FB filtration. Moreover, the hydrolysate of the collected cells could be successfully used as a nitrogen source to replace 50% yeast extract for decreasing the DC production cost. With repeated-batch culture in a membrane bioreactor, the maximal DC productivity could be enhanced by 57.8% compared with the batch culture, meanwhile n-dodecane and cells could be recovered and used for the next fermentation cycle.
本研究旨在利用十二烷和 Candida 细胞水解产物作为底物，通过膜集成重复分批发酵，开发一种生产α，ω-十二烷二酸（DC）的有效方法。在 4m/s 的错流速度和 0.2MPa 的跨膜压力下，通过 150kDa 陶瓷膜过滤发酵液（FB）时，同时回收细胞和十二烷，并且发现 FB 过滤过程中，发酵液中的细胞可以减轻膜污染。此外，收集的细胞水解产物可以成功用作氮源，替代 50%的酵母提取物，以降低 DC 的生产成本。在膜生物反应器中进行重复分批培养时，与分批培养相比，最大 DC 生产力提高了 57.8%，同时可以回收十二烷和细胞，用于下一个发酵周期。
