Research Department for Limnology, University of Innsbruck, Mondseestraße 9, 5310, Mondsee, Austria.
Research Department for Limnology, University of Innsbruck, Mondseestraße 9, 5310, Mondsee, Austria.
Water Res. 2018 Jul 1;138:234-240. doi: 10.1016/j.watres.2018.03.052. Epub 2018 Mar 23.
We combined profiling of the bloom-forming and potentially toxigenic cyanobacterium Planktothrix rubescens using a multiparameter probe equipped with a phycoerythrin sensor (in vivo fluorometry, IVL) in Lake Mondsee, Austria, with flow cytometric live analyses of discrete samples taken from several depths in the upper 20 m of the water column. Results obtained by IVL and acoustic flow cytometry (AFC) were compared to microscopic analyses of integrated (0-21 m) water samples using fixed material. This comparison was made because the integrated samples are used for the Austrian monitoring programme according to the EU Water Framework Directive. We demonstrate that AFC provides quantitative analyses of the filaments of P. rubescens that are significantly correlated to IVF and microscopic analyses, allowing rapid (within hours) and more precise calculation of P. rubescens biomass than estimates derived from IVL. Our analysis shows that vertically integrated water samples provide unreliable information on the concentration of P. rubescens in the upper surface waters and on the peak concentration of P. rubescens within the water column. We conclude that the protocol that we developed is superior to the current monitoring practice.
我们在奥地利蒙德湖（Lake Mondsee），结合配备藻红蛋白传感器的多参数探头对形成水华且具有潜在产毒能力的蓝藻束丝藻（Planktothrix rubescens）进行了分析（活体荧光计法，IVF），并对水柱上层 20 米范围内的多个深度采集的离散样本进行了流式细胞术的活体分析。IVL 和声学流动细胞术（AFC）的结果与使用固定材料对整合（0-21 米）水样的显微镜分析进行了比较。之所以进行这种比较，是因为根据欧盟水框架指令，整合样本被用于奥地利的监测计划。我们证明 AFC 可对束丝藻的丝状藻体进行定量分析，与 IVF 和显微镜分析具有显著相关性，可在数小时内快速（within hours）且更精确地计算束丝藻的生物量，优于 IVF 衍生的估计值。我们的分析表明，垂直整合的水样无法提供有关上覆水体中束丝藻浓度和水柱内束丝藻浓度峰值的可靠信息。我们得出的结论是，我们开发的方案优于当前的监测实践。
