注意：消除光片荧光成像中的条纹伪影。

Note: Eliminating stripe artifacts in light-sheet fluorescence imaging.

作者信息

Salili S M, Harrington M, Durian D J

机构信息

Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

出版信息

Rev Sci Instrum. 2018 Mar;89(3):036107. doi: 10.1063/1.5016546.

DOI:10.1063/1.5016546

PMID:29604752

Abstract

We report two techniques to mitigate stripe artifacts in light-sheet fluorescence imaging. The first uses an image processing algorithm called the multidirectional stripe remover method to filter stripes from an existing image. The second uses an elliptical holographic diffuser with strong scattering anisotropy to prevent stripe formation during image acquisition. These techniques facilitate accurate interpretation of image data, especially in denser samples. They are also facile and cost-effective.

摘要

我们报告了两种减轻光片荧光成像中条纹伪影的技术。第一种技术使用一种名为多方向条纹去除法的图像处理算法，从现有图像中滤除条纹。第二种技术使用具有强散射各向异性的椭圆形全息漫射器，以防止在图像采集过程中形成条纹。这些技术有助于准确解读图像数据，尤其是在密度较大的样本中。它们操作简便且具有成本效益。