Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Unidad Cuajimalpa, Avenida Vasco de Quiroga 4871, Col. Santa Fe Cuajimalpa. Delegación Cuajimalpa de Morelos, Ciudad de México, Mexico; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain.
Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Unidad Cuajimalpa, Avenida Vasco de Quiroga 4871, Col. Santa Fe Cuajimalpa. Delegación Cuajimalpa de Morelos, Ciudad de México, Mexico.
J Environ Manage. 2018 Jul 1;217:247-252. doi: 10.1016/j.jenvman.2018.03.097. Epub 2018 Apr 5.
This study aimed at systematically comparing the potential of two empirical methods for the estimation of the volumetric CH mass transfer coefficient (ka), namely gassing-out and oxygen transfer rate (OTR), to describe CH biodegradation in a fermenter operated with a methanotrophic consortium at 400, 600 and 800 rpm. The ka estimated from the OTR methodology accurately predicted the CH elimination capacity (EC) under CH mass transfer limiting conditions regardless of the stirring rate (∼9% of average error between empirical and estimated ECs). Thus, empirical CH-ECs of 37.8 ± 5.8, 42.5 ± 5.4 and 62.3 ± 5.2 g CH m hvs predicted CH-ECs of 35.6 ± 2.2, 50.1 ± 2.3 and 59.6 ± 3.4 g CH m h were recorded at 400, 600 and 800 rpm, respectively. The rapid Co-catalyzed reaction of O with SO in the vicinity of the gas-liquid interphase during OTR determinations, mimicking microbial CH uptake in the biotic experiments, was central to accurately describe the ka.
本研究旨在系统比较两种经验方法（鼓泡法和氧转移速率法）估算体积 CH 传质系数（ka）的潜力，以描述在 400、600 和 800 rpm 下以甲烷氧化菌共生物为基础的发酵罐中 CH 生物降解的情况。OTR 方法估算的 ka 能够准确预测 CH 消除容量（EC），即使在 CH 传质受限条件下，也不受搅拌速率的影响（经验和估计的 EC 之间的平均误差约为 9%）。因此，在 400、600 和 800 rpm 下，分别记录到的 37.8±5.8、42.5±5.4 和 62.3±5.2 g CH m hvs 的经验性 CH-ECs，预测的 CH-ECs 为 35.6±2.2、50.1±2.3 和 59.6±3.4 g CH m h。在 OTR 测定过程中，SO 在气液界面附近与 O 的快速协催化反应模拟了生物实验中微生物对 CH 的吸收，这对准确描述 ka 至关重要。
