Shi Wei, Liu Ying, Meng Qinghong, Yuan Lin, Gao Wei, Yao Kaihu
Capital Medical University, National Center for Children's Health, Beijing Children's Hospital, Beijing Pediatric Research Institute, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Ministry of Education, Key Laboratory of Major Diseases in Children, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing, China.
Capital Medical University, National Center for Children's Health, Beijing Children's Hospital, Beijing Pediatric Research Institute, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Ministry of Education, Key Laboratory of Major Diseases in Children, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing, China.
Braz J Microbiol. 2018 Oct-Dec;49(4):891-899. doi: 10.1016/j.bjm.2018.02.004. Epub 2018 Mar 14.
This study examined the antimicrobial susceptibility patterns and clonal complex (CC) characteristics of serogroup 6 Streptococcus pneumoniae isolates collected from children in Beijing, China, between 1997 and 2016. Serotypes were determined using the Quellung reaction, and the antimicrobial susceptibility profiles of the isolates were determined using the disc-diffusion method or by E-test. Sequence types (STs) were assigned based on multilocus sequence typing. A total of 250 isolates were examined, with 55.2%, 30.0%, 12.8%, and 2.0% of isolates identified as serotypes 6A, 6B, 6C, and 6D, respectively. All of the isolates were susceptible to levofloxacin and vancomycin, and the non-suceptibitility rate to penicillin was 41.6%. Eighty-two distinct STs, assigned to 13 CCs and 28 singletons, were identified. CC982 was the most prevalent CC amongst serotype 6A isolates (34%), followed by CC9789 and CC3173. Amongst serotype 6B isolates, CC90 and CC4542 were the most common, accounting for 25.3% and 14.7% of isolates respectively. Over the study period, the prevalence of CC982, CC4542, and CC4536 isolates showing susceptibility to penicillin and cefuroxime decreased, and the proportion of CC3173, CC9789, CC855, and CC902 isolates showing non-susceptibility to these two antibiotics increased.
本研究调查了1997年至2016年间从中国北京儿童中分离出的6型肺炎链球菌菌株的抗菌药物敏感性模式和克隆复合体（CC）特征。使用荚膜肿胀反应确定血清型，采用纸片扩散法或E试验确定菌株的抗菌药物敏感性谱。基于多位点序列分型确定序列类型（STs）。共检测了250株菌株，其中分别有55.2%、30.0%、12.8%和2.0%的菌株被鉴定为6A、6B、6C和6D血清型。所有菌株对左氧氟沙星和万古霉素敏感，对青霉素的不敏感率为41.6%。鉴定出82种不同的STs，分为13个CCs和28个单倍型。CC982是6A血清型菌株中最常见的CC（34%），其次是CC9789和CC3173。在6B血清型菌株中，CC90和CC4542最常见，分别占菌株的25.3%和14.7%。在研究期间，对青霉素和头孢呋辛敏感的CC982、CC4542和CC4536菌株的流行率下降，而对这两种抗生素不敏感的CC3173、CC9789、CC855和CC902菌株的比例增加。
