• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

λ整合酶基因的突变分析：显性突变的DNA序列

Mutational analysis of the lambda int gene: DNA sequence of dominant mutations.

作者信息

Bear S E, Clemens J B, Enquist L W, Zagursky R J

机构信息

Department of Molecular Biology, E. I. du Pont de Nemours & Co., Inc., Wilmington, Delaware 19898.

出版信息

J Bacteriol. 1987 Dec;169(12):5880-3. doi: 10.1128/jb.169.12.5880-5883.1987.

DOI:10.1128/jb.169.12.5880-5883.1987
PMID:2960665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214194/
Abstract

We have combined techniques of genetic and physical mapping with rapid DNA sequence analysis to identify the nucleotide change in lambda int mutations. These mutations define two dominant phenotypic classes: (i) recombination that is partially independent of accessory factors, and (ii) inhibition of wild-type Int by missense or nonsense proteins, i.e., negative complementation.

摘要

我们将遗传图谱和物理图谱技术与快速DNA序列分析相结合，以鉴定λ整合酶（Int）突变中的核苷酸变化。这些突变定义了两个主要的表型类别：（i）部分独立于辅助因子的重组，以及（ii）错义或无义蛋白对野生型Int的抑制作用，即负互补。

相似文献

1
Mutational analysis of the lambda int gene: DNA sequence of dominant mutations.λ整合酶基因的突变分析：显性突变的DNA序列
J Bacteriol. 1987 Dec;169(12):5880-3. doi: 10.1128/jb.169.12.5880-5883.1987.
2
Integration and excision of bacteriophage lambda: the mechanism of conservation site specific recombination.噬菌体λ的整合与切除：保守位点特异性重组机制
Annu Rev Genet. 1981;15:143-67. doi: 10.1146/annurev.ge.15.120181.001043.
3
Site-specific recombination functions of bacteriophage lambda: DNA sequence of regulatory regions and overlapping structural genes for Int and Xis.噬菌体λ的位点特异性重组功能：Int和Xis调控区及重叠结构基因的DNA序列
Proc Natl Acad Sci U S A. 1980 May;77(5):2482-6. doi: 10.1073/pnas.77.5.2482.
4
Features of bacteriophage lambda: analysis of the complete nucleotide sequence.λ噬菌体的特征：完整核苷酸序列分析
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1009-24. doi: 10.1101/sqb.1983.047.01.115.
5
Lambda site-specific recombination: the att site.λ位点特异性重组：附着位点
Cell. 1981 Sep;25(3):585-6. doi: 10.1016/0092-8674(81)90165-3.
6
DNA sequence of regulatory region for integration gene of bacteriophage lambda.噬菌体λ整合基因调控区的DNA序列
Proc Natl Acad Sci U S A. 1980 May;77(5):2477-81. doi: 10.1073/pnas.77.5.2477.
7
lambda altSF: a phage variant that acquired the ability to substitute specific sets of genes at high frequency.λ替代特异性因子（altSF）：一种噬菌体变体，它获得了在高频下替换特定基因集的能力。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):410-4. doi: 10.1073/pnas.78.1.410.
8
Structure and function of the phage lambda att site: size, int-binding sites, and location of the crossover point.噬菌体λ附着位点的结构与功能：大小、整合酶结合位点及交叉点位置
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:429-37. doi: 10.1101/sqb.1981.045.01.057.
9
[Deletion of late genes of the prophage lambda].[噬菌体λ前噬菌体晚期基因的缺失]
Mol Biol (Mosk). 1985 Nov-Dec;19(6):1457-65.
10
Multilevel regulation of bacteriophage lambda lysogeny by the E. coli himA gene.大肠杆菌himA基因对噬菌体λ溶原性的多级调控
Cell. 1981 Jul;25(1):269-76. doi: 10.1016/0092-8674(81)90252-x.

引用本文的文献

1
Receipt of the C-terminal tail from a neighboring lambda Int protomer allosterically stimulates Holliday junction resolution.来自相邻λ整合酶原体的C末端尾巴的接收会变构刺激霍利迪连接体的拆分。
J Mol Biol. 2005 Sep 2;351(5):948-55. doi: 10.1016/j.jmb.2005.06.077.
2
Two structural features of lambda integrase that are critical for DNA cleavage by multimers but not by monomers.λ整合酶的两个结构特征对于多聚体而非单体进行DNA切割至关重要。
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2770-5. doi: 10.1073/pnas.0400135101. Epub 2004 Feb 19.
3
Synaptic intermediates in bacteriophage lambda site-specific recombination: integrase can align pairs of attachment sites.噬菌体λ位点特异性重组中的突触中间体：整合酶可使附着位点对排列对齐。
EMBO J. 1993 Dec;12(12):4567-76. doi: 10.1002/j.1460-2075.1993.tb06145.x.
4
An intermediate in the phage lambda site-specific recombination reaction is revealed by phosphorothioate substitution in DNA.噬菌体λ位点特异性重组反应中的一个中间体通过DNA中的硫代磷酸酯取代得以揭示。
Nucleic Acids Res. 1988 Jul 25;16(14B):6839-56. doi: 10.1093/nar/16.14.6839.
5
A point mutation in the Nul gene of bacteriophage lambda facilitates phage growth in Escherichia coli with himA and gyrB mutations.噬菌体λ的Nul基因中的一个点突变促进了噬菌体在具有himA和gyrB突变的大肠杆菌中的生长。
Mol Gen Genet. 1988 Apr;212(1):149-56. doi: 10.1007/BF00322458.
6
Nucleotide sequence and genetic characterization of staphylococcal bacteriophage L54a int and xis genes.葡萄球菌噬菌体L54a整合酶基因和切除酶基因的核苷酸序列及遗传特征
J Bacteriol. 1989 Aug;171(8):4146-53. doi: 10.1128/jb.171.8.4146-4153.1989.
7
Integration host factor of Escherichia coli reverses the inhibition of R6K plasmid replication by pi initiator protein.大肠杆菌整合宿主因子可逆转π起始蛋白对R6K质粒复制的抑制作用。
J Bacteriol. 1991 Feb;173(3):1279-86. doi: 10.1128/jb.173.3.1279-1286.1991.

本文引用的文献

1
Integration and excision of bacteriophage lambda: the mechanism of conservation site specific recombination.噬菌体λ的整合与切除：保守位点特异性重组机制
Annu Rev Genet. 1981;15:143-67. doi: 10.1146/annurev.ge.15.120181.001043.
2
Structure and function of the phage lambda att site: size, int-binding sites, and location of the crossover point.噬菌体λ附着位点的结构与功能：大小、整合酶结合位点及交叉点位置
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:429-37. doi: 10.1101/sqb.1981.045.01.057.
3
The mechanism of phage lambda site-specific recombination: site-specific breakage of DNA by Int topoisomerase.λ噬菌体位点特异性重组的机制：Int拓扑异构酶对DNA的位点特异性切割
Cell. 1983 Dec;35(3 Pt 2):795-803. doi: 10.1016/0092-8674(83)90112-5.
4
Strand exchange in lambda integrative recombination: genetics, biochemistry, and models.λ整合重组中的链交换：遗传学、生物化学及模型
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:417-28. doi: 10.1101/sqb.1981.045.01.056.
5
An E. coli gene product required for lambda site-specific recombination.λ位点特异性重组所需的一种大肠杆菌基因产物。
Cell. 1980 Jul;20(3):711-9. doi: 10.1016/0092-8674(80)90317-7.
6
Integration-negative (int) mutants of phage lambda.噬菌体λ的整合阴性（int）突变体。
Virology. 1967 Jan;31(1):189. doi: 10.1016/0042-6822(67)90030-x.
7
Integration-negative mutants of bacteriophage lambda.噬菌体λ的整合阴性突变体
J Mol Biol. 1968 Feb 14;31(3):487-505. doi: 10.1016/0022-2836(68)90423-3.
8
Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi.噬菌体λ的缺失突变体。3. att-phi的物理结构。
J Mol Biol. 1971 Mar 14;56(2):403-23. doi: 10.1016/0022-2836(71)90473-6.
9
Nonessential functions of bacteriophage lambda.噬菌体λ的非必需功能。
Virology. 1969 Feb;37(2):177-88. doi: 10.1016/0042-6822(69)90197-4.
10
Mutants of bacteriophage lambda unable to integrate into the host chromosome.无法整合到宿主染色体中的噬菌体λ突变体。
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1507-14. doi: 10.1073/pnas.58.4.1507.