λ整合酶基因的突变分析：显性突变的DNA序列
Mutational analysis of the lambda int gene: DNA sequence of dominant mutations.
作者信息
Bear S E, Clemens J B, Enquist L W, Zagursky R J
机构信息
Department of Molecular Biology, E. I. du Pont de Nemours & Co., Inc., Wilmington, Delaware 19898.
出版信息
J Bacteriol. 1987 Dec;169(12):5880-3. doi: 10.1128/jb.169.12.5880-5883.1987.
Abstract
We have combined techniques of genetic and physical mapping with rapid DNA sequence analysis to identify the nucleotide change in lambda int mutations. These mutations define two dominant phenotypic classes: (i) recombination that is partially independent of accessory factors, and (ii) inhibition of wild-type Int by missense or nonsense proteins, i.e., negative complementation.
摘要
我们将遗传图谱和物理图谱技术与快速DNA序列分析相结合，以鉴定λ整合酶（Int）突变中的核苷酸变化。这些突变定义了两个主要的表型类别：（i）部分独立于辅助因子的重组，以及（ii）错义或无义蛋白对野生型Int的抑制作用，即负互补。
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本文引用的文献
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Annu Rev Genet. 1981;15:143-67. doi: 10.1146/annurev.ge.15.120181.001043.
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Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:429-37. doi: 10.1101/sqb.1981.045.01.057.
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Cell. 1983 Dec;35(3 Pt 2):795-803. doi: 10.1016/0092-8674(83)90112-5.
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Cell. 1980 Jul;20(3):711-9. doi: 10.1016/0092-8674(80)90317-7.
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