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对L.和L.的丙酮提取物和甲醇提取物的成分及抗氧化活性的研究

Investigation of the composition and antioxidant activity of acetone and methanol extracts of L. and L.

作者信息

Tongur Timur, Erkan Naciye, Ayranci Erol

机构信息

Department of Chemistry, Faculty of Science, Akdeniz University, 07058 Antalya, Turkey.

出版信息

J Food Sci Technol. 2018 Apr;55(4):1396-1406. doi: 10.1007/s13197-018-3054-9. Epub 2018 Feb 5.

Abstract

The compositions of methanol and acetone extracts obtained from L. and L. were investigated. The antioxidant activities of each extract were determined by various test methods. Phenolic composition profile analysis by HPLC-DAD showed that extracts contain more phenolic compounds than extracts. Among the components, syringic acid was found to be the most abundant one in extracts (42.8 and 38.4 mg per g dry extract of methanol and acetone, respectively). Total flavonoid, total phenolic and total carotenoid contents of methanolic extracts were found to be 244.5 mg rutin/g dry weight of extract, 1219.3 mg GAE/g dry weight of extract and 11.9 mg/g dry weight of extract, respectively. DPPH, ABTS and O assays were applied to plant extracts as radical scavenging activity tests. Methanolic extracts of . and . showed the highest radical scavenging activities according to DPPH and ABTS tests (61.6 and 147.2 in terms of IC, respectively). Antioxidant activity tests for measuring lipid oxidation inhibiting capacity were carried in low density lipoprotein (LDL) and bovine brain extract (BBE). Methanolic extracts of and demonstrated remarkable lipid oxidation inhibiting capacity in LDL and BBE tests.

摘要

对从[植物名称1]和[植物名称2]中获得的甲醇提取物和丙酮提取物的成分进行了研究。通过各种测试方法测定了每种提取物的抗氧化活性。采用高效液相色谱-二极管阵列检测器(HPLC-DAD)进行酚类成分谱分析,结果表明[植物名称1]提取物比[植物名称2]提取物含有更多的酚类化合物。在这些成分中,丁香酸被发现是[植物名称1]提取物中含量最丰富的一种(甲醇提取物和丙酮提取物中每克干提取物分别为42.8毫克和38.4毫克)。甲醇提取物中总黄酮、总酚和总类胡萝卜素的含量分别为244.5毫克芦丁/克提取物干重、1219.3毫克没食子酸乙酯/克提取物干重和11.9毫克/克提取物干重。采用DPPH、ABTS和氧自由基吸收能力(ORAC)测定法对植物提取物进行自由基清除活性测试。根据DPPH和ABTS测试,[植物名称1]和[植物名称2]的甲醇提取物表现出最高的自由基清除活性(IC50值分别为61.6和147.2)。在低密度脂蛋白(LDL)和牛脑提取物(BBE)中进行了测量脂质氧化抑制能力的抗氧化活性测试。[植物名称1]和[植物名称2]的甲醇提取物在LDL和BBE测试中表现出显著的脂质氧化抑制能力。

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