Gao Fangluan, Du Zhenguo, Shen Jianguo, Yang Hongkai, Liao Furong
Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Inspection and Quarantine Technology Center, Fujian Exit-Entry, Inspection and Quarantine Bureau, Fuzhou, Fujian, China.
PeerJ. 2018 Mar 27;6:e4550. doi: 10.7717/peerj.4550. eCollection 2018.
(OrMV) has a wide host range and affects the production of a variety of ornamentals. In this study, the coat protein (CP) gene of OrMVwas used to investigate the molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two groups which have significant subpopulation differentiation with an value of 0.470. One isolate was identified as a recombinant and the other 35 recombination-free isolates could be divided into two major clades under different evolutionary constraints with N/S values of 0.055 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In addition, the results from analysis of molecular variance (AMOVA) indicated that the effect of host species on the genetic divergence of OrMV is greater than that of geography. Furthermore, OrMV isolates from the genera and tended to group together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation.
木槿花叶病毒(OrMV)宿主范围广泛,会影响多种观赏植物的生产。在本研究中,利用木槿花叶病毒的外壳蛋白(CP)基因来探究该病毒进化的分子机制。36个木槿花叶病毒分离株分为两组,其亚群体分化显著,Fst值为0.470。鉴定出一个重组株,其他35个无重组的分离株可分为两个主要分支,处于不同的进化限制之下,N/S值分别为0.055和0.028,表明纯化选择在木槿花叶病毒分化中起作用。此外,分子方差分析(AMOVA)结果表明,宿主物种对木槿花叶病毒遗传分化的影响大于地理因素。此外,来自[具体属名1]属和[具体属名2]属的木槿花叶病毒分离株倾向于聚集在一起,表明木槿花叶病毒的多样化部分是由宿主驱动的适应性维持的。