Xie Lixue, Gao Fangluan, Shen Jianguo, Zhang Xiaoyan, Zheng Shan, Zhang Lijie, Li Tao
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China.
Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China.
PeerJ. 2020 Feb 21;8:e8576. doi: 10.7717/peerj.8576. eCollection 2020.
Telosma mosaic virus (TeMV) is an important plant virus causing considerable economic losses to passion fruit () production worldwide, including China. In this study, the complete genome sequence (excluding the poly (A) tail) of two TeMV isolates, Fuzhou and Wuyishan, were determined to be 10,050 and 10,057 nucleotides, respectively. Sequence analysis indicated that Fuzhou and Wuyishan isolates share 78-98% nucleotide and 83-99% amino acid sequence identities with two TeMV isolates of Hanoi and GX, and a proposed new potyvirus, tentatively named PasFru. Phylogenetic analysis indicated that these TeMV isolates and PasFru were clustered into a monophyletic clade with high confidences. This indicated that PasFru and the four TeMV isolates should be considered as one potyvirus species. Two recombination breakpoints were identified within the CI and NIb genes of the Fuzhou isolate, and also within the P1 gene of the Wuyishan isolate. To the best of our knowledge, this is the first report of TeMV recombinants worldwide.
夜来香花叶病毒（TeMV）是一种重要的植物病毒，在包括中国在内的全球范围内给百香果生产造成了相当大的经济损失。在本研究中，福州和武夷山两个TeMV分离株的完整基因组序列（不包括多聚（A）尾）分别确定为10,050和10,057个核苷酸。序列分析表明，福州和武夷山分离株与河内和GX的两个TeMV分离株以及一种暂定名为PasFru的新马铃薯Y病毒属病毒共享78-98%的核苷酸和83-99%的氨基酸序列同一性。系统发育分析表明，这些TeMV分离株和PasFru被高度置信地聚类到一个单系分支中。这表明PasFru和这四个TeMV分离株应被视为一个马铃薯Y病毒属病毒物种。在福州分离株的CI和NIb基因内以及武夷山分离株的P1基因内鉴定出两个重组断点。据我们所知，这是全球范围内关于TeMV重组体的首次报道。
