He Jie-Tao, Huang Hong-Yan, Qu Dong, Xue Ye, Zhang Kai-Kai, Xie Xiao-Li, Wang Qi
Department of Forensic Pathology, School of Forensic Medicine, Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou, Guangdong, China.
Department of Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou, Guangdong, China.
Forensic Sci Med Pathol. 2018 Jun;14(2):174-179. doi: 10.1007/s12024-018-9969-7. Epub 2018 Apr 2.
Detection of the vitality of wounds is one of the most important issues in forensic practice. This study investigated mRNA and protein levels of CXCL1 and CXCR2 in skin wounds in mice and humans. Western blot analysis of CXCL1 and CXCR2 protein levels showed no difference between wounded and intact skin. However, mRNA levels demonstrated higher expression of CXCL1 and CXCR2 in contused mouse and human skin, compared with intact skin. At postmortem there were no remarkable changes in CXCL1 and CXCR2 mRNA levels in contused mouse skin. Increased mRNA expression was observed in contused mouse skin up to 96 h and 72 h after death for CXCL1 and CXCR2 respectively. In human samples of wounded skin, increased CXCL1 mRNA levels were detected up to 48 h after autopsy in all 5 cases, while increased CXCR2 mRNA levels were observed 48 h after autopsy in 4 of 5 cases. These findings suggest that the levels of CXCL1 and CXCR2 mRNA present in contused skin can be used as potential markers for a vital reaction in forensic practice.
伤口活力的检测是法医实践中最重要的问题之一。本研究调查了小鼠和人类皮肤伤口中CXCL1和CXCR2的mRNA和蛋白质水平。CXCL1和CXCR2蛋白质水平的蛋白质印迹分析显示，受伤皮肤和完整皮肤之间没有差异。然而，与完整皮肤相比，mRNA水平显示挫伤的小鼠和人类皮肤中CXCL1和CXCR2的表达更高。死后，挫伤的小鼠皮肤中CXCL1和CXCR2 mRNA水平没有明显变化。分别在死亡后96小时和72小时，在挫伤的小鼠皮肤中观察到CXCL1和CXCR2的mRNA表达增加。在人类受伤皮肤样本中，所有5例在尸检后48小时内均检测到CXCL1 mRNA水平升高，而5例中有4例在尸检后48小时观察到CXCR2 mRNA水平升高。这些发现表明，挫伤皮肤中存在的CXCL1和CXCR2 mRNA水平可作为法医实践中生命反应的潜在标志物。
