Ishida Yuko, Kimura Akihiko, Nosaka Mizuho, Kuninaka Yumi, Shimada Emi, Yamamoto Hiroki, Nishiyama Kosuke, Inaka Shogo, Takayasu Tatsunori, Eisenmenger Wolfgang, Kondo Toshikazu
Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, 641-8509, Wakayama, Japan.
Int J Legal Med. 2015 Sep;129(5):1049-54. doi: 10.1007/s00414-015-1181-7. Epub 2015 Apr 7.
Endothelial progenitor cells (EPCs), a newly identified cell type, are bone marrow-derived progenitor cells that co-express stem cell markers and vascular endothelial growth factor (VEGF) receptor (Flk-1). In this study, a double-color immunofluorescence analysis was carried out using anti-CD34 and anti-Flk-1 antibodies to examine the time-dependent appearance of EPCs, using 52 human skin wounds with different wound ages (Group I, 0-1 days; Group II, 2-6 days; Group III, 7-14 days; and Group IV, 17-21 days). In wound specimens with an age of less than one day, CD34(+)/Flk-1(+) EPCs were not detected. EPCs were initially observed in wounds aged two days, and their number was increased in lesions with advances in wound age. In morphometrical analysis, the average number of EPCs was the highest in the wounds of Group III. Especially, 20 out of 21 wounds aged 7-12 days had >20 EPCs, and all wound samples with postinfliction intervals of 14-21 days had <15 EPCs. These observations at least showed that >20 EPCs would indicate a wound age of 7-12 days. Taken together, our observations indicate the detection of EPCs would be useful for wound age determination.
内皮祖细胞（EPCs）是一种新发现的细胞类型，是骨髓来源的祖细胞，共表达干细胞标志物和血管内皮生长因子（VEGF）受体（Flk-1）。在本研究中，使用抗CD34和抗Flk-1抗体进行双色免疫荧光分析，以检测EPCs随时间的出现情况，研究对象为52例不同伤口年龄的人类皮肤伤口（I组，0-1天；II组，2-6天；III组，7-14天；IV组，17-21天）。在伤口年龄小于1天的标本中，未检测到CD34(+)/Flk-1(+) EPCs。EPCs最初在2天龄的伤口中被观察到，并且随着伤口年龄的增加，其数量在损伤部位增多。在形态计量分析中，III组伤口中EPCs的平均数量最高。特别是，21例7-12天龄的伤口中有20例EPCs数量>20，而所有受伤后间隔为14-21天的伤口样本中EPCs数量<15。这些观察结果至少表明，EPCs数量>20表明伤口年龄为7-12天。综上所述，我们的观察结果表明，检测EPCs对伤口年龄的确定是有用的。
