Rudd C E, Morimoto C, Wong L L, Schlossman S F
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
J Exp Med. 1987 Dec 1;166(6):1758-73. doi: 10.1084/jem.166.6.1758.
The T4 (CD4) subset of T lymphocytes has been subdivided into two major subsets, a suppressor/inducer subset (T4+,2H4+) and a helper subset (T4+,2H4-) on the basis of the differential expression of the L-C/T200 (CD45) antigens. The 2H4 antigen itself comprises at least three distinct polypeptides at 125,200, and 220 X 10(3) Mr, of which the 200 and 220 X 10(3) Mr polypeptides constitute the highest Mr isoforms of a pool of five distinct L-C/T200 antigens. The T4+,2H4+ subset expresses at least four of these isoforms at 180, 190, 200, and 220 X 10(3) on the cell surface, while the T4+,2H4- subset expresses only the 180 and 190 X 10(3) Mr forms. Pulse-chase analysis and endoglycosidase treatment revealed that the 125 X 10(3) Mr chain of the 2H4 antigen is nonglycosylated, while the 200 and 220 X 10(3) polypeptides are structurally related and derived by N- and O-linked glycosylation from two nascent subunits at 150 and 160 X 10(3) Mr. The function of the T4+,2H4+ subset could be blocked only by an antibody reactive with the L-C/T200 isoforms enriched with O-linked oligosaccharides at 200 and 220 X 10(3) Mr.
根据L-C/T200（CD45）抗原的差异表达，T淋巴细胞的T4（CD4）亚群已被细分为两个主要亚群，即抑制/诱导亚群（T4 +，2H4 +）和辅助亚群（T4 +，2H4 -）。2H4抗原本身至少由三种不同的多肽组成，分子量分别为125、200和220×10³Mr，其中200和220×10³Mr的多肽构成了五种不同的L-C/T200抗原池中的最高分子量同工型。T4 +，2H4 +亚群在细胞表面表达至少四种这些同工型，分子量分别为180、190、200和220×10³，而T4 +，2H4 -亚群仅表达180和190×10³Mr的形式。脉冲追踪分析和内切糖苷酶处理表明，2H4抗原的125×10³Mr链是非糖基化的，而200和220×10³的多肽在结构上相关，并且是由150和160×10³Mr的两个新生亚基通过N-和O-连接的糖基化衍生而来。T4 +，2H4 +亚群的功能只能被一种与200和220×10³Mr富含O-连接寡糖的L-C/T200同工型反应的抗体所阻断。
