Dental Biomaterials, Preventive & Minimally Invasive Dentistry, Departamento de Odontologia, CEU Carndenal Herrera University, Valencia, Spain; Tissue Engineering and Biophotonics Research Division, King's College London Dental Institute, King's College London, United Kingdom.
Tissue Engineering and Biophotonics Research Division, King's College London Dental Institute, King's College London, United Kingdom.
J Dent. 2018 Jun;73:32-39. doi: 10.1016/j.jdent.2018.03.014. Epub 2018 Mar 30.
To evaluate the effect of load-cycle aging and/or 6 months artificial saliva (AS) storage on bond durability and interfacial ultramorphology of resin-modified glass ionomer cement (RMGIC) applied onto dentine air-abraded using Bioglass 45S5 (BAG) with/without polyacrylic acid (PAA) conditioning.
RMGIC (Ionolux, VOCO) was applied onto human dentine specimens prepared with silicon-carbide abrasive paper or air-abraded with BAG with or without the use of PAA conditioning. Half of bonded-teeth were submitted to load cycling (150,000 cycles) and half immersed in deionised water for 24 h. They were cut into matchsticks and submitted immediately to microtensile bond strength (μTBS) testing or 6 months in AS immersion and subsequently μTBS tested. Results were analysed statistically by two-way ANOVA and Student-Newman-Keuls test (α = 0.05). Fractographic analysis was performed using FE-SEM, while further RMGIC-bonded specimens were surveyed for interfacial ultramorphology characterisation (dye-assisted nanoleakage) using confocal microscopy.
RMGIC applied onto dentine air-abraded with BAG regardless PAA showed no significant μTBS reduction after 6 months of AS storage and/or load cycling (p > 0.05). RMGIC-dentine interface showed no sign of degradation/nanoleakage after both aging regimens. Conversely, interfaces created in PAA-conditioned SiC-abraded specimens showed significant reduction in μTBS (p < 0.05) after 6 months of storage and/or load cycling with evident porosities within bonding interface.
Dentine pre-treatment using BAG air-abrasion might be a suitable strategy to enhance the bonding performance and durability of RMGIC applied to dentine. The use of PAA conditioner in smear layer-covered dentine may increase the risk of degradation at the bonding interface.
A combined dentine pre-treatment using bioglass followed by PAA may increase the bond strength and maintain it stable over time. Conversely, the use of PAA conditioning alone may offer no significant contribute to the immediate and prolonged bonding performance.
评估负载循环老化和/或 6 个月人工唾液（AS）储存对应用于经 Bioglass 45S5（BAG）喷砂处理并用/不用聚丙烯酸（PAA）预处理牙本质的树脂改良型玻璃离子水门汀（RMGIC）的粘结耐久性和界面超微形态的影响。
将 RMGIC（Ionolux，VOCO）应用于用碳化硅砂纸制备或用 BAG 喷砂处理并用/不用 PAA 预处理的人牙本质标本上。一半的粘结牙接受 150000 次循环负载，另一半浸入去离子水中 24 小时。将它们切成火柴棒，立即进行微拉伸粘结强度（μTBS）测试或在 AS 中浸泡 6 个月后进行随后的 μTBS 测试。结果通过双因素方差分析和 Student-Newman-Keuls 检验（α=0.05）进行统计学分析。使用 FE-SEM 进行断口分析，同时使用共聚焦显微镜对进一步的 RMGIC 粘结标本进行界面超微形态特征（染料辅助纳米渗漏）调查。
无论是否使用 PAA，应用于经 BAG 喷砂处理的牙本质的 RMGIC 在 6 个月的 AS 储存和/或负载循环后，μTBS 均无显著降低（p>0.05）。在两种老化方案后，RMGIC-牙本质界面均未显示出降解/纳米渗漏的迹象。相反，在 PAA 预处理的 SiC 喷砂处理标本中形成的界面在储存和/或负载循环 6 个月后 μTBS 显著降低（p<0.05），粘结界面内可见明显的孔隙。
使用 BAG 喷砂处理牙本质的预处理可能是增强应用于牙本质的 RMGIC 粘结性能和耐久性的合适策略。在覆盖有玷污层的牙本质中使用 PAA 调节剂可能会增加粘结界面降解的风险。
使用生物玻璃联合牙本质预处理，然后使用 PAA，可提高粘结强度并使其随时间保持稳定。相反，单独使用 PAA 预处理可能对即刻和长期粘结性能没有显著贡献。
