Children's Hospital of Soochow University, Pediatric Research Institute of Soochow University, Suzhou, Jiangsu 215123, China.
Nanoscale. 2018 Apr 19;10(15):6981-6991. doi: 10.1039/c8nr00838h.
Nanotechnology-mediated anti-inflammatory therapy is emerging as a novel strategy for the treatment of inflammation-induced injury. However, one of the main hurdles for these anti-inflammatory nano-drugs is their potential toxic side effects in vivo. Herein, we uncovered that polydopamine (PDA) nanoparticles with their structure and chemical properties similar to melanin, a natural bio-polymer, displayed a significant anti-inflammation therapeutic effect on acute inflammation-induced injury. PDA with enriched phenol groups functioned as a radical scavenger to eliminate reactive oxygen species (ROS) generated during inflammatory responses. As revealed by in vivo photoacoustic imaging with a H2O2-specific nanoprobe, PDA nanoparticles remarkably reduced intracellular ROS levels in murine macrophages challenged with either H2O2 or lipopolysaccharide (LPS). The anti-inflammatory capacity of PDA nanoparticles was further demonstrated in murine models of both acute peritonitis and acute lung injury (ALI), where diminished ROS generation, reduced proinflammatory cytokines, attenuated neutrophil infiltration, and alleviated lung tissue damage were observed in PDA-treated mice after a single dose of PDA treatment. Our work therefore presents the great promise of PDA nanoparticles as a biocompatible nano-drug for anti-inflammation therapy to treat acute inflammation-induced injury.
纳米技术介导的抗炎疗法作为一种治疗炎症诱导损伤的新策略正在兴起。然而，这些抗炎纳米药物的主要障碍之一是它们在体内的潜在毒副作用。本文中，我们发现结构和化学性质类似于黑色素的聚多巴胺（PDA）纳米颗粒作为一种天然生物聚合物，对急性炎症诱导的损伤具有显著的抗炎治疗作用。富含酚基团的 PDA 作为一种自由基清除剂，可消除炎症反应过程中产生的活性氧（ROS）。通过使用 H2O2 特异性纳米探针进行体内光声成像显示，PDA 纳米颗粒可显著降低受到 H2O2 或脂多糖（LPS）刺激的小鼠巨噬细胞内的 ROS 水平。在急性腹膜炎和急性肺损伤（ALI）的小鼠模型中进一步证实了 PDA 纳米颗粒的抗炎能力，在单次 PDA 治疗后，PDA 处理的小鼠中观察到 ROS 生成减少、促炎细胞因子减少、中性粒细胞浸润减轻和肺组织损伤缓解。因此，我们的工作表明 PDA 纳米颗粒作为一种用于治疗急性炎症诱导损伤的抗炎治疗的生物相容纳米药物具有巨大的应用前景。
