Teagasc Food Research Programme, Moorepark, Fermoy, Co. Cork, Ireland.
Department of Microbiology, University College Cork, Cork, Ireland.
Eur J Pediatr. 2018 Jun;177(6):867-870. doi: 10.1007/s00431-018-3133-y. Epub 2018 Apr 2.
The aim of this study was to determine bacteriological stability of a probiotic mixture dispersed in various diluents. The commercially available probiotic (Infloran®), containing Bifidobacterium bifidum (10 CFU/250 mg tablet) and Lactobacillus acidophilus (10 CFU/250 mg tablet), was dispersed within expressed breast milk, sterile water, and infant formula and examined at temperatures of 4 and 21 °C. When stored at 4 °C, significant decreases (P < 0.05) in the level of L. acidophilus and B. bifidum were observed in expressed breast milk and sterile water after a 6-h period. However, when stored in infant formula, both strains remained stable over a 12-h period. When stored at 21 °C, a significant decrease (P < 0.05) was observed in the level of L. acidophilus in sterile water, expressed breast milk and infant formula throughout a 12-h period. However, no significant decrease was observed overtime in B. bifidum in all three diluents at this temperature.
Our findings suggest that, when stored at 4 °C, this probiotic product can remain at a stable condition for 6 h in sterile water and infant formula; however, the viability of the probiotic decreases significantly after this period of time. Administration of this probiotic in sterile water can be an acceptable alternative to dispersion and administration in expressed breast milk. What is Known: • Administration of probiotics containing lactobacilli and bifidobacteria has become more widespread in neonatology, mainly as prophylaxis for the prevention of necrotising entercolitis in preterm infants. • Probiotic reconstitution, from its powder base, is not standardized and various diluents, including sterile water, breast milk, and infant formula, have been used. What is New: • When stored at 4 °C, a probiotic containing lactobacilli and bifidobacteria remains at a stable microbological condition for up to 6 h in sterile water. • Administration of this probiotic dispersed in sterile water, followed by an EBM feed, can be an acceptable alternative to dispersion and administration in EBM.
本研究旨在确定分散在不同稀释剂中的益生菌混合物的细菌学稳定性。市售益生菌（Infloran®）含有双歧杆菌（10 CFU/250 mg 片剂）和嗜酸乳杆菌（10 CFU/250 mg 片剂），分别分散在母乳、无菌水和婴儿配方奶粉中，在 4 和 21°C 下进行检测。在 4°C 下储存时，在 6 小时后，母乳和无菌水中的嗜酸乳杆菌和双歧杆菌水平明显下降（P<0.05）。然而，当在婴儿配方奶粉中储存时，两种菌株在 12 小时内保持稳定。在 21°C 下储存时，在 12 小时内，无菌水、母乳和婴儿配方奶粉中嗜酸乳杆菌水平显著下降（P<0.05）。然而，在该温度下，在所有三种稀释剂中，双歧杆菌的数量在整个时间内均无明显下降。
我们的研究结果表明，当在 4°C 下储存时，该益生菌产品在无菌水和婴儿配方奶粉中可在 6 小时内保持稳定状态；然而，在此时间段后，益生菌的活力会显著下降。在无菌水中给予该益生菌可作为在母乳中分散和给药的替代方法。
·含乳杆菌和双歧杆菌的益生菌的应用在新生儿学中越来越广泛，主要作为预防早产儿坏死性小肠结肠炎的预防措施。
·益生菌的再配制，从其粉末基础开始，尚未标准化，并且已经使用了各种稀释剂，包括无菌水、母乳和婴儿配方奶粉。
·当在 4°C 下储存时，含有乳杆菌和双歧杆菌的益生菌在无菌水中可在稳定的微生物条件下保存长达 6 小时。
·在无菌水中分散该益生菌，然后进行 EBM 喂养，可作为在 EBM 中分散和给药的替代方法。
