Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
Department of Microbiology, University College Cork, Cork, Ireland.
Arch Dis Child Fetal Neonatal Ed. 2019 Mar;104(2):F159-F164. doi: 10.1136/archdischild-2017-313468. Epub 2018 Jun 20.
The objective of this study was to investigate the appropriate dosing interval of a probiotic (Infloran) given daily, biweekly and weekly in preterm infants <32 weeks' gestation.
There were 8 infants in the daily group, 8 infants in the biweekly group and 10 infants in the weekly group, all born between 25 and 32 weeks' gestation. The control group consisted of 12 preterm infants who did not receive the probiotic. Infloran (250 mg/capsule), containing (1×10 colony-forming unit (CFU)) and (1×10 CFU), was administered in 2.5 mL of breast milk per kilogram weight of the infant (2×10 CFU of bacteria in total), until 34 weeks postmenstrual age (PMA). Stool samples were collected at 31, 34, 41 and 44 weeks PMA and frozen at -20°C.
After administration of the probiotic at 31 weeks PMA, were significantly higher in the daily group (45%) in comparison with the biweekly (17%) and weekly (9%) groups. At 34 weeks PMA, were significantly higher again in the daily (60%) group in comparison with the biweekly (21%), weekly (23%) and control (15%) groups. At 41 weeks PMA a decrease in the relative abundances of Streptococcaceae and Enterococcaceae was found in all three probiotic groups, and by 44 weeks PMA significantly higher levels of were found in the biweekly group (16.5%) in comparison with the weekly group (2.1%).
Our results indicate that a daily dose of Infloran is a suitable dosage for preterm infants in the neonatal intensive care unit, with significantly higher levels of found in the daily probiotic group up to 44 weeks PMA.
本研究旨在探讨每日、每两周和每周给予早产儿（<32 周）益生菌（Infloran）的适当剂量间隔。
每日组有 8 名婴儿，每两周组有 8 名婴儿，每周组有 10 名婴儿，均出生于 25 至 32 周妊娠。对照组由 12 名未接受益生菌的早产儿组成。Infloran（250mg/胶囊），含有 （1×10 菌落形成单位（CFU））和 （1×10 CFU），按婴儿体重每公斤 2.5mL 给予母乳（总细菌数为 2×10 CFU），直至出生后 34 周（周龄）。在 31、34、41 和 44 周 PMA 时收集粪便样本并在-20°C 下冷冻。
在 31 周 PMA 时给予益生菌后，每日组（45%）的 显著高于每两周组（17%）和每周组（9%）。在 34 周 PMA 时，每日组（60%）再次显著高于每两周组（21%）、每周组（23%）和对照组（15%）。在 41 周 PMA 时，所有三个益生菌组中的链球菌科和肠球菌科的相对丰度均下降，到 44 周 PMA 时，每周组（2.1%）显著高于每周组（16.5%）。
我们的结果表明，每日剂量的 Infloran 是新生儿重症监护病房早产儿的合适剂量，在 44 周 PMA 时，每日益生菌组的 水平显著更高。
