一种新型体外DNA包装系统，证明了噬菌体λ FI基因产物的直接作用。
A novel in vitro DNA packaging system demonstrating a direct role for the bacteriophage lambda FI gene product.
作者信息
Davidson A, Gold M
机构信息
Department of Medical Genetics, University of Toronto, Ontario, Canada.
出版信息
Virology. 1987 Dec;161(2):305-14. doi: 10.1016/0042-6822(87)90122-x.
A new in vitro bacteriophage lambda DNA packaging system is described in which all the proteins necessary for head morphogenesis are supplied by extracts of plasmid-transformed cells. This assay is used to demonstrate that the lambda FI gene product (gpFI) is necessary for maximal packaging efficiency when proheads and terminase are present in limiting amounts. A 100- to 200-fold decrease in packaging is seen when gpFI is omitted. gpFI is shown to act at and/or after the stage in packaging where proheads bind to the DNA:terminase complex.
本文描述了一种新的体外噬菌体λ DNA 包装系统，其中头部形态发生所需的所有蛋白质均由质粒转化细胞的提取物提供。该检测方法用于证明，当原头部和末端酶的量有限时，λ FI 基因产物（gpFI）对于最大包装效率是必需的。当省略 gpFI 时，包装效率会降低 100 至 200 倍。结果表明，gpFI 在原头部与 DNA：末端酶复合物结合的包装阶段及之后发挥作用。
Zotero 插件