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一种用于体外λ噬菌体DNA包装的特定系统。

A defined system for in vitro lambda DNA packaging.

作者信息

Hwang Y, Feiss M

机构信息

Department of Microbiology, University of Iowa, Iowa City 52242, USA.

出版信息

Virology. 1995 Aug 20;211(2):367-76. doi: 10.1006/viro.1995.1419.

DOI:10.1006/viro.1995.1419
PMID:7645241
Abstract

We constructed a defined in vitro system for packaging lambda DNA which is composed of purified proheads, the noncapsid proteins terminase and gpFI, and the Escherichia coli DNA binding/bending protein IHF. The defined packaging system: (i) is free from endogenous ATP, DNAs, and DNases and (ii) packages 30% of the input mature lambda DNA efficiently. In this defined packaging system, IHF and gpFI make modest contributions to packaging efficiency. The defined packaging reactions showed that DNA packaging gave a linear response to the concentration of mature lambda DNA and terminase. DNA packaging showed a sigmoidal relationship with respect to the concentration of ATP and proheads.

摘要

我们构建了一个用于包装λ DNA的体外确定系统，该系统由纯化的原头部、非衣壳蛋白末端酶和gpF1以及大肠杆菌DNA结合/弯曲蛋白IHF组成。该确定的包装系统：（i）不含内源性ATP、DNA和DNA酶，（ii）能有效包装30%的输入成熟λ DNA。在这个确定的包装系统中，IHF和gpF1对包装效率的贡献不大。确定的包装反应表明，DNA包装对成熟λ DNA和末端酶的浓度呈线性响应。DNA包装对ATP和原头部的浓度呈S形关系。

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A defined system for in vitro lambda DNA packaging.一种用于体外λ噬菌体DNA包装的特定系统。
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2
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