大鼠接触水烟筒滤水后，其骨髓细胞会出现染色体损伤。

Chromosomal damage at bone-marrow cells is induced by exposure of rats to waterpipe water filtrate.

作者信息

Azab Mohammad A, Khabour Omar F, Alzoubi Karem H, Alzubi Mohammad A, Masadeh Majed M, Shakhatreh Muhamad Ali K

机构信息

a Department of Basic Medical Sciences, Faculty of Medicine , The Hashemite University , Zarqa , Jordan.

b Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences , Jordan University of Science and Technology , Irbid , Jordan.

出版信息

Drug Chem Toxicol. 2018 Oct;41(4):394-398. doi: 10.1080/01480545.2018.1452933. Epub 2018 Apr 3.

DOI:10.1080/01480545.2018.1452933

PMID:29611445

Abstract

Waterpipe smoking is continuing to spread globally. The aim of this study is to investigate the effect of waterpipe water filtrate on chromosomal integrity in the bone-marrow cells of rats. Chromosomal damage was examined using in vivo chromosomal aberrations (CAs) and SCEs assays. Young Wistar male rats were exposed to WWF via drinking water. Chromosomal damage was measured in bone marrow cells after 6 weeks of treatment using fluorescent-plus-Giemsa staining. Treatment of rats with waterpipe water filtrate for 6 weeks did not affect food/liquid consumption and gain in body weight. The results showed that waterpipe water filtrate increased the frequencies of chromosomal breaks and exchanges by more than 30% (p < 0.01). In addition, waterpipe water filtrate significantly increased SCEs in the bone-marrow cells of rats. In conclusion, waterpipe water filtrate contains genotoxic compounds providing additional evidence for genotoxicity of waterpipe smoke.

摘要

水烟吸食正在全球范围内持续蔓延。本研究旨在调查水烟过滤水对大鼠骨髓细胞染色体完整性的影响。使用体内染色体畸变（CAs）和姐妹染色单体交换（SCEs）试验检测染色体损伤。将年轻的雄性Wistar大鼠通过饮用水暴露于水烟过滤水。在治疗6周后，使用荧光加吉姆萨染色法测量骨髓细胞中的染色体损伤。用水烟过滤水对大鼠进行6周治疗并不影响食物/液体消耗和体重增加。结果表明，水烟过滤水使染色体断裂和交换频率增加了30%以上（p < 0.01）。此外，水烟过滤水显著增加了大鼠骨髓细胞中的SCEs。总之，水烟过滤水含有遗传毒性化合物，为水烟烟雾的遗传毒性提供了额外证据。