Wang J, Liu M, Bai X, Zhang H, Xu Z, Zhao D, Zhao Y
Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, 1035 Boshuo Road, Changchun, Jilin, People's Republic of China.
Pol J Vet Sci. 2017 Dec;20(4):751-758. doi: 10.1515/pjvs-2017-0095.
Thymosin beta 10 (Tβ10) is a member of the β-thymosin family. As an actin-binding peptide, thymosin β10 is involved in many important biological activities. Transcriptome sequencing results suggest that Tβ10 may play important roles in the growth of deer antler. In this study, Tβ10 cDNA was isolated from sika deer, and complete open reading frame consisting of 129 nucleotides was obtained by PCR amplification. The predicted peptide was 42 amino acids in length. The sdTβ10 cDNA was cloned and expressed in Escherichia coli resulting in a 6 kDa recombinant-His tagged protein. The recombinant, non-glycosylated peptide was overexpressed in a soluble form and purified by immobilized metal ion affinity chromatography. Functional studies revealed that recombinant Tβ10 stimulated osteoblasts proliferation. This study provides the first evidence that recombinant sika deer Tβ10 promotes proliferation in an osteoblasts cell model.
胸腺素β10（Tβ10）是β-胸腺素家族的成员。作为一种肌动蛋白结合肽，胸腺素β10参与许多重要的生物学活动。转录组测序结果表明，Tβ10可能在鹿茸生长中发挥重要作用。在本研究中，从梅花鹿中分离出Tβ10 cDNA，并通过PCR扩增获得了由129个核苷酸组成的完整开放阅读框。预测的肽长度为42个氨基酸。将梅花鹿Tβ10 cDNA克隆并在大肠杆菌中表达，产生了一种6 kDa的重组His标签蛋白。重组的非糖基化肽以可溶形式过度表达，并通过固定化金属离子亲和色谱法纯化。功能研究表明，重组Tβ10刺激成骨细胞增殖。本研究提供了首个证据，即重组梅花鹿Tβ10在成骨细胞模型中促进增殖。
