Abacioğlu N, Ercan Z S, Kanzik L, Zengil H, Demiryürek T, Türker R K
Department of Pharmacology, Faculty of Pharmacy, Gazi University, Etiler/Ankara.
Agents Actions. 1987 Oct;22(1-2):30-5. doi: 10.1007/BF01968813.
Contribution of endothelial cells (ECs) to the effects of histamine (HA) was investigated on the isolated guinea-pig main pulmonary artery (GPPA) strips precontracted with noradrenaline (NA). HA caused dose-dependent relaxation at the concentrations ranged between 10(-8) to 10(-6) M but produced a contraction when a relatively higher concentration (10(-5) M) was used in unrubbed strips. Cimetidine partially inhibited the relaxing effect of HA without altering its constrictive action. In rubbed strips, however, HA produced a dose-dependent contraction. The constrictive effect of HA in rubbed strips enhanced after addition of cimetidine to the incubation medium. HA elicited a concentration-dependent relaxation in both unrubbed and rubbed strips in the presence of mepyramine. Impromidine produced a relaxation in the strips with and without endothelium. These data was taken as an evidence indicating that HA caused a relaxation in the isolated GPPA strips, first causing the release of endothelium derived relaxing factor (EDRF) which is triggered by H1-receptors and secondly by the direct stimulation of H2-receptors.
研究了内皮细胞（ECs）对组胺（HA）作用的影响，实验对象为用去甲肾上腺素（NA）预收缩的豚鼠离体主肺动脉（GPPA）条。HA在10^(-8)至10^(-6) M浓度范围内引起剂量依赖性舒张，但在未摩擦的条带中使用相对较高浓度（10^(-5) M）时则产生收缩。西咪替丁部分抑制HA的舒张作用，而不改变其收缩作用。然而，在摩擦的条带中，HA产生剂量依赖性收缩。在孵育培养基中加入西咪替丁后，HA在摩擦条带中的收缩作用增强。在存在美吡拉敏的情况下，HA在未摩擦和摩擦的条带中均引起浓度依赖性舒张。英普咪定在有和没有内皮的条带中均产生舒张作用。这些数据表明，HA在离体GPPA条带中引起舒张，首先是通过H1受体触发内皮衍生舒张因子（EDRF）的释放，其次是通过直接刺激H2受体。
