采用响应面法从铁皮石斛中提取和分析巨豆三醇。
Extraction and Analysis of Gigantol from Dendrobium officinale with Response Surface Methodology.
机构信息
College of Life Science, Anhui Agricultural University, Hefei 230036, China.
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
出版信息
Molecules. 2018 Apr 3;23(4):818. doi: 10.3390/molecules23040818.
In order to optimize the extraction of gigantol from , the influence of methanol concentration, ultrasonic temperature, and liquid ratio on extraction efficiency was analysed by the response surface analysis method. The results show that the extraction rate reached a maximum when the methanol concentration was 92.98%, the solid-liquid ratio was 27.2 mL/g, and the extraction temperature was 41.41 °C. The content of gigantol of in leaves was significantly higher than that in stems, reaching 4.7942 μg/g. The content of gigantol in Fengdou was significantly higher than that of other species of Fengdou. This experiment has practical significance for improving the utilization rate of , and provides a reference for the study of the pharmacological and biological activity of gigantol.
为了优化从 中提取 gigantol 的条件，采用响应面分析法分析了甲醇浓度、超声温度和液比对提取效率的影响。结果表明，当甲醇浓度为 92.98%、固液比为 27.2 mL/g、提取温度为 41.41°C 时，提取率达到最大值。叶片 gigantol 的含量明显高于茎部，达到 4.7942 μg/g。丰都 的 gigantol 含量明显高于其他丰都种。该实验对提高 的利用率具有实际意义，为 gigantol 的药理和生物学活性研究提供了参考。
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