College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Public Administration, Nanjing Agricultural University, Nanjing 210095, China.
Molecules. 2017 Dec 18;22(12):2250. doi: 10.3390/molecules22122250.
This work provides an optimized extraction approach intended to maximize the recovery of dihydromyricetin (DHM) from Chinese vine tea () leaves. The presented work adopts a Box-Behnken design as a response surface methodology to understand the role and influence of specific extraction parameters including: time, temperature, and solvent composition/ethanol (%) on DHM final yields. Initially, single factor experiments were used to delineate the role of above factors (temperature, time, and solvent composition) before proceeding with three factors-three levels Box-Behnken design with 17 separate runs to assess the effect of multifactorial treatments on DHM recovery rates. The collected data shows that independent variables (solvent composition, time, and temperature) can significantly affect DHM recovery rates with maximum yields resulting from a combined 60 °C, 60% aqueous ethanol, and 180 min treatment. From the empirical point of view, the above optimized extraction protocol can substantially enhance processing and profitability margins with a minimum need of interventions or associated costs.
本研究旨在提供一种优化的提取方法，以最大限度地从中国藤茶（）叶中提取二氢杨梅素（DHM）。本研究采用 Box-Behnken 设计作为响应面法，以了解特定提取参数（包括时间、温度和溶剂组成/乙醇（%））对 DHM 最终产率的作用和影响。首先，采用单因素实验来描绘上述因素（温度、时间和溶剂组成）的作用，然后进行三因素三水平 Box-Behnken 设计，进行 17 次独立运行，以评估多因素处理对 DHM 回收率的影响。收集的数据表明，自变量（溶剂组成、时间和温度）可以显著影响 DHM 的回收率，最高产率来自于 60°C、60%水乙醇和 180 分钟的组合处理。从经验角度来看，上述优化的提取方案可以显著提高加工和盈利的利润率，而所需的干预或相关成本最小。
