Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, Julius-von-Sachs Platz 2, 97082 Würzburg, Germany.
Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, Julius-von-Sachs Platz 2, 97082 Würzburg, Germany.
Mol Plant. 2018 Jun 4;11(6):764-775. doi: 10.1016/j.molp.2018.03.017. Epub 2018 Mar 31.
Plants and animals in endosomes operate TPC1/SV-type cation channels. All plants harbor at least one TPC1 gene. Although the encoded SV channel was firstly discovered in the plant vacuole membrane two decades ago, its biological function has remained enigmatic. Recently, the structure of a plant TPC1/SV channel protein was determined. Insights into the 3D topology has now guided site-directed mutation approaches, enabling structure-function analyses of TPC1/SV channels to shed new light on earlier findings. Fou2 plants carrying a hyperactive mutant form of TPC1 develop wounding stress phenotypes. Recent studies with fou2 and mutants that lack functional TPC1 have revealed atypical features in local and long-distance stress signaling, providing new access to the previously mysterious biology of this vacuolar cation channel type in planta.
内体中的植物和动物都有 TPC1/SV 型阳离子通道。所有植物至少都有一个 TPC1 基因。尽管编码的 SV 通道在二十年前首次在植物液泡膜中被发现，但它的生物学功能仍然是个谜。最近，植物 TPC1/SV 通道蛋白的结构被确定。对 3D 拓扑结构的深入了解现在指导了定点突变方法，使 TPC1/SV 通道的结构-功能分析能够为早期的发现提供新的见解。携带 TPC1 超活性突变形式的 fou2 植物会表现出创伤应激表型。最近对 fou2 和缺乏功能 TPC1 的突变体的研究揭示了局部和长距离应激信号传递中的非典型特征，为以前神秘的液泡阳离子通道类型在植物体内的生物学提供了新的研究途径。
