Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, C1121ABG Ciudad Autónoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1425FQB Ciudad Autónoma de Buenos Aires, Argentina.
Instituto de Investigaciones Científicas y Técnicas para la Defensa (CITEDEF), B1603ALQ Ciudad Autónoma de Buenos Aires, Argentina.
Ecotoxicol Environ Saf. 2018 Aug 15;157:121-127. doi: 10.1016/j.ecoenv.2018.03.046. Epub 2018 Mar 31.
Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were tested in culture peripheral blood of armadillo Chaetophractus villosus with cytogenetic biomarkers like mitotic index (MI), chromosomal aberrations (CA), sister chromatid exchange (SCE) and cell proliferation kinetics (CPK) by means of replication index. Adults animals of both sexes were exposed to RU at four concentrations ranging from 0.026 mL RU solution to 0.379 mL RU daily in oral treatment with the same volume (0.2 mL) during 7 days. We analyzed the induced damage at different times considering T0 as control value, one (T1), seven (T7) and 30 days (T30). One day after, only the higher concentration shows MI significant differences (p < 0.05), at T7 the frequency increases and at T30 it decreases reaching T0 values. The analysis of CA frequencies shows that only 0.106 mL RU/day exhibit significant differences vs T0 values. A great variability is expressed in the values of standard deviation (SD) and in the wide confidence intervals of the media. One day after treatments (T1) all four concentrations shows significant differences in SCE vs T0 values. Replication Index (RI) does not show significant differences. The dose-response behavior was not observed in either CA or SCE. The consistency of the findings obtained with the same biomarkers in vitro support the idea of expanding studies in order to characterize the risk doses for these mammals.
不同浓度的草甘膦制剂，Roundup® Full II（66.2%草甘膦），在犰狳 Chaetophractus villosus 的外周血培养中，通过复制指数用有丝分裂指数（MI）、染色体畸变（CA）、姐妹染色单体交换（SCE）和细胞增殖动力学（CPK）等细胞遗传学生物标志物进行了测试。雄性和雌性成年动物分别以 4 种浓度（0.026 mL RU 溶液至 0.379 mL RU）进行 RU 暴露，每天口服治疗，体积相同（0.2 mL），持续 7 天。我们在不同时间分析了诱导损伤，将 T0 作为对照值，分别为 T1（暴露后 1 天）、T7（暴露后 7 天）和 T30（暴露后 30 天）。暴露后 1 天，只有较高浓度的草甘膦显示出 MI 有显著差异（p<0.05），在 T7 时频率增加，在 T30 时降低，达到 T0 值。CA 频率分析表明，只有 0.106 mL RU/天与 T0 值有显著差异。标准差（SD）和中值的置信区间的变异性很大。在 T1（暴露后 1 天），所有 4 种浓度的 SCE 与 T0 值相比均有显著差异。复制指数（RI）没有显示出显著差异。在 CA 或 SCE 中均未观察到剂量-反应关系。在体外使用相同生物标志物获得的研究结果的一致性支持了扩大研究以确定这些哺乳动物的危险剂量的想法。
