Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Jagiellonska 4, 41-200 Sosnowiec, Poland.
Department of Pathology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Ostrogórska 30, 41-200 Sosnowiec, Poland.
Molecules. 2018 Mar 31;23(4):805. doi: 10.3390/molecules23040805.
We have studied a preventive effect of polyphenol-rich bee pollen ethanol extract (EEP) against histological changes in the liver and cardiac blood vessels, abnormalities of lipid profile, and the levels of oxidized low density lipoproteins (ox-LDL), asymmetric dimethylarginine (ADMA), angiotensin-converting enzyme (ACE), and angiotensin II (ANG II) caused by a high-fat diet in CBL₆ mice. Supplementing the diet with EEP in the doses of 0.1 g/kg body mass (BM) and 1 g/kg BM resulted in a decrease of total cholesterol by 31% and 35%, respectively. It also decreased the level of low density lipoproteins by 67% and 90%, respectively. No differences in the levels of high density lipoprotein and triacylglycerols were observed. EEP reduced the level of ox-LDL by 33% and 47%, ADMA by 13% and 51%, ACE by 17% and 30%, as well as ANG II by 11% and 15% in a dose-dependent manner, which proves a protective effect of EEP in a high-fat diet. EEP reduces and/or prevents hepatic steatosis and degenerative changes caused by a high-fat diet in CBL₆ mice, which indicates its hepatoprotective effect. EEP used with standard feed does not disturb a normal concentration of the assayed parameters.
我们研究了富含多酚的蜂花粉乙醇提取物（EEP）对高脂肪饮食引起的 CBL₆ 小鼠肝脏和心血管组织形态学变化、脂质谱异常、氧化型低密度脂蛋白（ox-LDL）、不对称二甲基精氨酸（ADMA）、血管紧张素转换酶（ACE）和血管紧张素 II（ANG II）水平的预防作用。用 EEP 以 0.1 g/kg 体重（BM）和 1 g/kg BM 的剂量补充饮食，可使总胆固醇分别降低 31%和 35%。它还使低密度脂蛋白的水平分别降低了 67%和 90%。高密度脂蛋白和三酰甘油的水平没有差异。EEP 以剂量依赖的方式使 ox-LDL 降低了 33%和 47%，ADMA 降低了 13%和 51%，ACE 降低了 17%和 30%，ANG II 降低了 11%和 15%，证明了 EEP 在高脂肪饮食中的保护作用。EEP 减少和/或预防了高脂肪饮食引起的 CBL₆ 小鼠肝脂肪变性和退行性变化，表明其具有肝保护作用。EEP 与标准饲料一起使用不会干扰所测参数的正常浓度。
