Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University, Jinju 52828, Korea.
College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China.
Molecules. 2018 Apr 2;23(4):816. doi: 10.3390/molecules23040816.
s Merr. et Rolfe has been cultivated on a large scale and is widely consumed by local inhabitants as an important nutraceutical, especially against rheumatism which has a deep connection with antioxidants. In this study, a total of 18 different phenolic metabolite compounds in s were isolated and identified, and evaluated for their antioxidant and DNA damage protection potential. The antioxidant activity of the 18 identified compounds was screened using DPPH, ORAC, hydroxyl and superoxide radical scavenging assays. The antioxidant potential of the compounds was found to differ by functionality and skeleton. However, most compounds showed a good antioxidant potential. In particular, seven of the identified compounds, namely, compounds , , , , , and , showed significant protective effects on pBR322 plasmid DNA against the mutagenic and toxic effects of Fenton's reaction. The most active compound, compound , displayed a dose-dependent DNA damage protection potential in the range of 7.560.0 μM. The DNA damage protective effect of the identified compounds was significantly correlated with the hydroxyl radical scavenging activity. Compounds that exhibited effective (IC = 5.412.5 μg/mL) hydroxyl radical scavenging activity were found to be the ones with higher DNA damage protection potential.
益智 Merr. et Rolfe 已被大规模种植，被当地居民广泛用作一种重要的营养保健品，特别是针对与抗氧化剂密切相关的风湿病。在这项研究中，共分离鉴定了益智中的 18 种不同的酚类代谢物化合物，并评估了它们的抗氧化和 DNA 损伤保护潜力。使用 DPPH、ORAC、羟基和超氧自由基清除测定法筛选了 18 种鉴定化合物的抗氧化活性。发现化合物的抗氧化潜力因功能和骨架而异。然而，大多数化合物表现出良好的抗氧化潜力。特别是，在所鉴定的七种化合物中，化合物 、 、 、 、 、 和 ，对 pBR322 质粒 DNA 表现出对 Fenton 反应致突变和毒性的显著保护作用。最活跃的化合物 ，在 7.560.0 μM 的范围内表现出剂量依赖性的 DNA 损伤保护潜力。所鉴定化合物的 DNA 损伤保护作用与羟基自由基清除活性显著相关。表现出有效（IC = 5.412.5 μg/mL）羟基自由基清除活性的化合物被发现具有更高的 DNA 损伤保护潜力。
