a College of TCM , Xinjiang Medical University , Urumqi , China.
b Department of TCM , The First Affiliated Hospital of the Medical College, Shihezi University , Shihezi , China.
Nat Prod Res. 2019 Sep;33(17):2549-2553. doi: 10.1080/14786419.2018.1452002. Epub 2018 Apr 3.
This study investigated the flavonoid constituents of a traditional Chinese medical plant Lam. by using ultra-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry and screened the active components in regulating autophagy.Normal rat kidney (NRK) cells transfected with green fluorescent protein- microtubule-associated protein 1 light Chain 3(GFP-LC3) were treated with s flavonoids and its chemical compositions. After 4 h of treatment, the auto-phagy spot aggregation in NRK cells was photographed and observed by laser scanning confocal microscopy. The following 10 flavonoid components of were identified: baicalein(1), quercetin(2), hyperoside(3), quercetin3-O-β-d-glucopyranoside(4), apigenin(5), kaempferol(6), chrysin(7), diosimin(8), linarin(9) and rutin(10). Among these flavonoids, chrysin, apigenin and quercetin were identified as the active principles in activating autophagy. This research may provide a reference for further developing and utilizing
本研究采用超高效液相色谱-四极杆飞行时间串联质谱联用技术对传统中药 Lam. 的黄酮类成分进行了研究，筛选出了调节自噬的活性成分。将绿色荧光蛋白-微管相关蛋白 1 轻链 3（GFP-LC3）转染的正常大鼠肾（NRK）细胞用 s 黄酮及其化学成分进行处理。处理 4 小时后，通过激光共聚焦扫描显微镜观察 NRK 细胞中自噬斑点的聚集情况。鉴定出 10 种黄酮类成分：黄芩素（1）、槲皮素（2）、金丝桃苷（3）、槲皮素 3-O-β-d-吡喃葡萄糖苷（4）、芹菜素（5）、山柰酚（6）、白杨素（7）、地奥司明（8）、圣草酚（9）和芦丁（10）。其中，白杨素、芹菜素和槲皮素被鉴定为激活自噬的活性成分。本研究可为进一步开发利用提供参考。
